Evaluation of 2 real-time PCR assays for in vitro diagnostic use in the rapid and multiplex detection of EGFR gene mutations in NSCLC
| Autor: | WK Chan, Rene M.Y. To, Anthony T.C. Wong, Edmond S. K. Ma, Chris L. P. Wong |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Gene mutation Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine law.invention symbols.namesake T790M law Carcinoma Non-Small-Cell Lung medicine Humans Multiplex Pathology Molecular Molecular Biology Gene Polymerase chain reaction Aged Sanger sequencing Aged 80 and over Mutation Cell Biology Genes erbB-1 Middle Aged Molecular biology Real-time polymerase chain reaction symbols Costs and Cost Analysis Female |
| Zdroj: | Diagnostic molecular pathology : the American journal of surgical pathology, part B. 22(3) |
| ISSN: | 1533-4066 |
| Popis: | Activating mutations of the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer predict for a favorable clinical response to tyrosine kinase inhibitor therapy. Although Sanger sequencing is a conventional method to detect EGFR gene mutations, multiplex real-time allele-specific polymerase chain reaction (PCR) systems are increasingly used in the routine molecular diagnostic setting. We aim to evaluate 2 proprietary real-time PCR assays (cobas and therascreen) against Sanger sequencing in the detection of EGFR gene mutations. The overall concordance rate between cobas and therascreen assays with Sanger sequencing was 89% and 88%, respectively, and increased to 96% and 98%, respectively, if the mutations not covered were excluded. The cobas assay showed a superior coverage of exon 20 mutations, but L861Q was not targeted. The nature of specimen, DNA integrity, and tumor cell content are factors that affect the assay performance. DNA extracted from cell block and clot of pleural fluid gave rise to 1 invalid call and 1 false-negative result by the cobas assay and 1 missed T790M mutation and 1 false-negative result by the therascreen assay. Both assays are around 5 times more expensive compared with Sanger sequencing in terms of reagent cost. We conclude that both assays prove to be a rapid, simple, and validated method in detecting the most common and clinically significant EGFR gene mutations in non-small cell lung cancer. Although less convenient compared with real-time PCR assays, Sanger sequencing is cheaper in terms of reagent cost and allows the detection of rare or novel EGFR gene mutations. |
| Databáze: | OpenAIRE |
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