Existence of natural mouse IgG mAbs recognising epitopes shared by malondialdehyde acetaldehyde adducts and Porphyromonas gingivalis
| Autor: | Ulrich Bergmann, Ramin Akhi, Chunguang Wang, Antti E. Nissinen, Outi Kummu, Pirkko J. Pussinen, Heidi M. Kaski, Mikael Kyrklund, Sohvi Hörkkö |
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| Přispěvatelé: | Clinicum, Medicum, Department of Oral and Maxillofacial Diseases, Doctoral Programme in Oral Sciences, HUS Head and Neck Center |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
education Immunology Fimbria Virulence Biology Microbiology Epitope Periodontal pathogen 03 medical and health sciences 0302 clinical medicine malondialdehyde acetaldehyde periodontitis Molecular Biology Porphyromonas gingivalis Tissue homeostasis 11832 Microbiology and virology Innate immune system 030206 dentistry Cell Biology natural Ab biology.organism_classification Gingipain 030104 developmental biology Infectious Diseases 1182 Biochemistry cell and molecular biology atherosclerosis |
| Zdroj: | Innate Immunity. 27:158-169 |
| ISSN: | 1753-4267 1753-4259 |
| DOI: | 10.1177/1753425920981133 |
| Popis: | Natural Abs are produced by B lymphocytes in the absence of external Ag stimulation. They recognise self, altered self and foreign Ags, comprising an important first-line defence against invading pathogens and serving as innate recognition receptors for tissue homeostasis. Natural IgG Abs have been found in newborns and uninfected individuals. Yet, their physiological role remains unclear. Previously, no natural IgG Abs to oxidation-specific epitopes have been reported. Here, we show the cloning and characterisation of mouse IgG mAbs against malondialdehyde acetaldehyde (MAA)-modified low-density lipoprotein. Sequence analysis reveals high homology with germline genes, suggesting that they are natural. Further investigation shows that the MAA-specific natural IgG Abs cross-react with the major periodontal pathogen Porphyromonas gingivalis and recognise its principle virulence factors gingipain Kgp and long fimbriae. The study provides evidence that natural IgGs may play an important role in innate immune defence and in regulation of tissue homeostasis by recognising and removing invading pathogens and/or modified self-Ags, thus being involved in the development of periodontitis and atherosclerosis. |
| Databáze: | OpenAIRE |
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