A hemolytic polypeptide from the venom of the red harvester ant, Pogonomyrmex barbatus
| Autor: | Alan W. Bernheimer, Justin O. Schmidt, Lois S. Avigad |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Erythrocytes
Guinea Pigs Venom In Vitro Techniques Biology Toxicology Hemolysis Median lethal dose Lethal Dose 50 Hemolysin Proteins Mice Dogs Botany Animals Humans Horses Amino Acids Arthropod Venoms Sheep Ant Venoms Isoelectric focusing Goats Hemolysin Pogonomyrmex Red harvester ant biology.organism_classification Haemolysis Rats Isoelectric point Biochemistry Cattle Rabbits Isoelectric Focusing Peptides |
| Zdroj: | Toxicon. 18:271-278 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(80)90005-7 |
| Popis: | The venom of Pogonomyrmex barbatus was analyzed with a view to elucidating the chemical nature of the agent responsible for direct hemolysis. Gel filtration experiments indicated the mol. wt of the active substance, termed barbatolysin, to be ca. 3000. Barbatolysin was isolated in nearly pure form by isoelectric focusing and was found to be a strongly basic polypeptide containing 34 amino acid residues. The relative susceptibility of erythrocytes from different animals was compared using venoms of P. barbatus, P. badius and Apis mellifera. Barbatolysin was not lethal for mice, but mouse lethality was associated with a second venom component having an isoelectric pH of 8·0 and a mol. wt of 8000. The information obtained suggests that there is a functional resemblance between the venoms of P. barbatus and A. mellifera with respect to direct-acting hemolysins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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