Genetic variants in 3′-UTRs of methylenetetrahydrofolate reductase (MTHFR) predict colorectal cancer susceptibility in Koreans
| Autor: | Jung O Kim, Doyeun Oh, Hye Mi Park, Sung Won Kwon, Young Joo Jeon, Seong Gyu Hwang, Jong Woo Kim, Hyo Geun Jang, Nam Keun Kim, Jisu Oh |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Homocysteine Colorectal cancer Adenocarcinoma Polymorphism Single Nucleotide Article Metastasis chemistry.chemical_compound Folic Acid Gene Frequency Republic of Korea microRNA Genotype medicine TaqMan Humans Genetic Predisposition to Disease 3' Untranslated Regions Genotyping Genetic Association Studies Methylenetetrahydrofolate Reductase (NADPH2) Aged Genetics Multidisciplinary biology business.industry Middle Aged medicine.disease digestive system diseases chemistry Methylenetetrahydrofolate reductase biology.protein Cancer research Female Colorectal Neoplasms business |
| Zdroj: | SCIENTIFIC REPORTS(5) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep11006 |
| Popis: | Polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) play important roles in tumor development, progression and metastasis. Moreover, recent studies have reported that a number of 3′-UTR polymorphisms potentially bind to specific microRNAs in a variety of cancers. The aim of this study was to investigate the association of four MTHFR polymorphisms, 2572C>A [rs4846049], 4869C>G [rs1537514], 5488C>T [rs3737967] and 6685T>C [rs4846048] with colorectal cancer (CRC) in Koreans. A total of 850 participants (450 CRC patients and 400 controls) were enrolled in the study. The genotyping of MTHFR 3′-UTR polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism analysis or TaqMan allelic discrimination assay. We found that MTHFR 2572C>A, 4869C>G and 5488C>T genotypes were substantially associated with CRC susceptibility. Of the potentially susceptible polymorphisms, MTHFR 2572C>A was associated with increased homocysteine and decreased folate levels in the plasma based on MTHFR 677CC. Our study provides the evidences for 3′-UTR variants in MTHFR gene as potential biomarkers for use in CRC prevention. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|