Potential allergenicity research of Cry1C protein from genetically modified rice
| Autor: | Sishuo Cao, Kunlun Huang, Yunbo Luo, Yunqing Dai, Xiaoyun He, Wentao Xu, Wenjun Ran, Lixing Liang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Peanut agglutinin
Ovalbumin Acid Phosphatase Food Genetically Modified Enzyme-Linked Immunosorbent Assay Genetically modified crops Pharmacology Real-Time Polymerase Chain Reaction Toxicology Immunoglobulin E Peanut Agglutinin chemistry.chemical_compound Rats Inbred BN Escherichia coli medicine Animals Mast Cells Sensitization Plant Proteins biology Acid phosphatase Oryza General Medicine Plants Genetically Modified Genetically modified rice Rats Eosinophils medicine.anatomical_structure Biochemistry chemistry Immunoglobulin G biology.protein Cytokines Female Food Hypersensitivity Histamine |
| Zdroj: | Regulatory Toxicology and Pharmacology. 63:181-187 |
| ISSN: | 0273-2300 |
| Popis: | With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three other proteins to determine the potential allergenicity of Cry1C protein from genetically modified rice. Female Brown Norway (BN) rats received 0.1 mg peanut agglutinin (PNA), 1mg potato acid phosphatase (PAP), 1mg ovalbumin (OVA) or 5 mg purified Cry1C protein dissolved in 1 mL water by daily gavage for 42 days to test potential allergenicity. Ten days after the last gavage, rats were orally challenged with antigens, and physiologic and immunologic responses were studied. In contrast to sensitization with PNA, PAP and OVA Cry1C protein did not induce antigen-specific IgG2a in BN rats. Cytokine expression, serum IgE and histamine levels and the number of eosinophils and mast cells in the blood of Cry1C group rats were comparable to the control group rats, which were treated with water alone. As Cry1C did not show any allergenicity, we make the following conclusion that the protein could be safety used in rice or other plants. |
| Databáze: | OpenAIRE |
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