Whole-Genome Sequencing of Lactobacillus Helveticus D75 and D76 Confirms Safety and Probiotic Potential
| Autor: | O. N. Shalaeva, Elena Demyanova, Timur Vakhitov, V A Toropov, Stanislav Sitkin |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
pacbio biology_other Microbiology Genome Article bacteriocins DNA sequencing law.invention 03 medical and health sciences Probiotic Bacteriocin law Virology Lactobacillus lcsh:QH301-705.5 Gene 030304 developmental biology Whole genome sequencing 0303 health sciences whole genome sequencing Lactobacillus helveticus biology 030306 microbiology food and beverages biology.organism_classification probiotic genes lactobacillus helveticus probiotics lcsh:Biology (General) gene expression |
| Zdroj: | Microorganisms, Vol 8, Iss 3, p 329 (2020) Microorganisms Volume 8 Issue 3 |
| Popis: | Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the Lactobacillus helveticus, but not to the L. acidophilus, as previously assumed. In addition, 31 exopolysaccharide (EPS) production genes (nine of which form a single genetic cluster), 13 adhesion genes, 38 milk protein and 11 milk sugar utilization genes, 13 genes for and against specific antagonistic activity, eight antibiotic resistance genes, and also three CRISPR blocks and eight Cas I-B system genes were identified in the genomes of both strains. The expression of bacteriocin helveticin J genes was confirmed. In fact, the presence of identified genes suggests that L. helveticus D75 and D76 are able to form biofilms on the outer mucin layer, inhibit the growth of pathogens and pathobionts, utilize milk substrates with the formation of digestible milk sugars and bioactive peptides, resist bacteriophages, show some genome-determined resistance to antibiotics, and stimulate the host&rsquo s immune system. Pathogenicity genes have not been identified. The study results confirm the safety and high probiotic potential of the strains. |
| Databáze: | OpenAIRE |
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