Cloning and characterization of IL-17B and IL-17C, two new members of the IL-17 cytokine family
| Autor: | Jian Chen, Sherry Heldens, Arthur J Huang, Austin L. Gurney, Jessica Foster, Patrick Dowd, Jeremy Stinson, Hanzhong Li, William I. Wood |
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| Rok vydání: | 2000 |
| Předmět: |
Male
DNA Complementary Recombinant Fusion Proteins medicine.medical_treatment Molecular Sequence Data Gene Expression Biology Monocytes Cell Line Proinflammatory cytokine Cell surface receptor Cricetinae Gene expression medicine Animals Humans Protein Isoforms Tissue Distribution Amino Acid Sequence RNA Messenger Cloning Molecular Receptor Receptors Interleukin-17 Multidisciplinary Sequence Homology Amino Acid Tumor Necrosis Factor-alpha Interleukin-17 Chromosome Mapping Receptors Interleukin Sequence Analysis DNA Biological Sciences Molecular biology Recombinant Proteins IL 17 family Cytokine Chromosomes Human Pair 5 Cytokines Female Tumor necrosis factor alpha Interleukin 17 Sequence Alignment Chromosomes Human Pair 16 Interleukin-1 Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences. 97:773-778 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | IL-17 is a T cell-derived cytokine that may play an important role in the initiation or maintenance of the proinflammatory response. Whereas expression of IL-17 is restricted to activated T cells, the IL-17 receptor is found to be widely expressed, a finding consistent with the pleiotropic activities of IL-17. We have cloned and expressed two novel human cytokines, IL-17B and IL-17C, that are related to IL-17 ( approximately 27% amino acid identity). IL-17B mRNA is expressed in adult pancreas, small intestine, and stomach, whereas IL-17C mRNA is not detected by RNA blot hybridization of several adult tissues. No expression of IL-17B or IL-17C mRNA is found in activated T cells. In a survey of cytokine induction, IL-17B and IL-17C stimulate the release of tumor necrosis factor alpha and IL-1beta from the monocytic cell line, THP-1, whereas IL-17 has only a weak effect in this system. No induction of IL-1alpha, IL-6, IFN-gamma, or granulocyte colony-stimulating factor is found in THP-1 cells. Fluorescence-activated cell sorter analysis shows that IL-17B and IL-17C bind to THP-1 cells. Conversely, IL-17B and IL-17C are not active in an IL-17 assay or the stimulation of IL-6 release from human fibroblasts and do not bind to the human IL-17 receptor extracellular domain. These data show that there is a family of IL-17-related cytokines differing in patterns of expression and proinflammatory responses that may be transduced through a cognate set of cell surface receptors. |
| Databáze: | OpenAIRE |
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