Characterisation and Mutagenesis Study of An Alternative Sigma Factor Gene (hrpL) from Erwinia mallotivora Reveal Its Central Role in Papaya Dieback Disease
| Autor: | Amin Asyraf Tamizi, Aimera-Farhana Samsuddin, Abdul-Munir Abdul-Murad, Rohaiza Ahmad-Redzuan, Norliza Abu-Bakar, Lina Rozano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Erwinia transcriptome sequencing medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Sigma factor Phylogenetics medicine Coding region lcsh:QH301-705.5 Gene Escherichia coli Genetics Bacterial disease General Immunology and Microbiology biology Nucleic acid sequence Malaysia biology.organism_classification bacterial disease 030104 developmental biology papaya lcsh:Biology (General) General Agricultural and Biological Sciences |
| Zdroj: | Biology Volume 9 Issue 10 Biology, Vol 9, Iss 323, p 323 (2020) |
| ISSN: | 2079-7737 |
| Popis: | The alternative sigma (&sigma ) factor E, RpoE or HrpL, has been reported to be involved in stress- and pathogenicity-related transcription initiation in Escherichia coli and many other Gram-negative bacteria, including Erwinia spp. and Pseudomonas spp. A previous study identified the hrpL/rpoE transcript as one of the significant differentially expressed genes (DEGs) during early E. mallotivora infection in papaya and those data serve as the basis of the current project. Here, the full coding DNA sequence (CDS) of hrpL from E. mallotivora (EmhrpL) was determined to be 549 bp long, and it encoded a 21.3 kDa HrpL protein that possessed two highly conserved sigma-70 (&sigma 70) motifs&mdash &sigma R2 and &sigma R4. Nucleotide sequence alignment revealed the hrpL from E. mallotivora shared high sequence similarity to rpoE/hrpL from E. tracheiphila (83%), E. pyrifoliae (81%), and E. tasmaniensis (80%). Phylogenetics analysis indicated hrpL from E. mallotivora to be monophyletic with rpoEs/hrpLs from Pantoea vagans, E. herbicola, and E. tracheiphila. Structural analysis postulated that the E. mallotivora&rsquo s alternative &sigma factor was non-transmembranic and was an extracytoplasmic function (ECF) protein&mdash characteristics shared by other &sigma factors in different bacterial species. Notably, the protein&ndash protein interaction (PPI) study through molecular docking suggested the &sigma factor could be possibly inhibited by an anti-&sigma Finally, a knockout of hrpL in E. mallotivora (&Delta EmhrpL) resulted in avirulence in four-month-old papaya plants. These findings have revealed that the hrpL is a necessary element in E. mallotivora pathogenicity and also predicted that the gene can be inhibited by an anti-&sigma |
| Databáze: | OpenAIRE |
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