Angiogenic potential is reduced in skeletal muscle of aged women
| Autor: | Birgitte Hoier, Ylva Hellsten, Line Nørregaard Olsen, Camilla Vestergaard Hansen, M. Leinum, Jens Bangsbo, Tue Smith Jørgensen, Howard H. Carter |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A medicine.medical_specialty Physiology Neovascularization Physiologic 03 medical and health sciences chemistry.chemical_compound Basal (phylogenetics) 0302 clinical medicine Internal medicine medicine Myocyte Humans Muscle Skeletal Exercise Aged business.industry Skeletal muscle VO2 max Endothelial Cells Infant Middle Aged medicine.disease Capillaries Vascular endothelial growth factor Endothelial stem cell Menopause 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Ageing Female business 030217 neurology & neurosurgery |
| Zdroj: | The Journal of physiologyReferences. 598(22) |
| ISSN: | 1469-7793 |
| Popis: | KEY POINTS The risk of cardiovascular disease and associated skeletal muscle microvascular rarefaction is enhanced in women after menopause, yet knowledge about the angiogenic potential in ageing women is generally sparse. Aged healthy and sedentary women were found to present a markedly impaired capacity for proliferation of skeletal muscle derived microvascular endothelial cells compared to young women. Vascular endothelial growth factor (VEGF) levels in skeletal muscle myocytes and release of VEGF from myocytes tended to be lower in aged compared to young women. The aged women did not show a detectable increase in skeletal muscle capillarization with 8 weeks of intense aerobic cycle training. Combined, the findings indicate that aged women have a reduced potential for capillary growth in skeletal muscle which, with ageing, may lead to age-induced microvascular rarefaction. ABSTRACT Skeletal muscle angiogenic potential was examined in cell cultures derived from aged and young women, and the effect of 8 weeks of intense cycle training on muscle capillary growth was determined in the group of aged women. Basal muscle samples were obtained from healthy sedentary aged (n = 12; 64 ± 4.2 years) and young women (n = 5; 24 ± 3.2 years) for endothelial cell and skeletal muscle myocyte isolation and experiments. In addition, the aged women completed an 8-week training intervention. Peak oxygen uptake and muscle samples for histology and protein determination were obtained before and after the training period. Before training, muscle microdialysate was collected from the aged women at rest and during exercise. In Part 1 of the experiments, growth-supplement stimulated proliferation of endothelial cells was ∼75% lower in cells from aged compared to young women (P |
| Databáze: | OpenAIRE |
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