Comparison of the salivary and dentinal microbiome of children with severe-early childhood caries to the salivary microbiome of caries-free children
| Autor: | C. Anthony Ryan, Catherine Stanton, Paul W. O'Toole, Eimear Hurley, Maurice P. J. Barrett, Martin Kinirons, Hugh M. B. Harris, Helen Whelton |
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| Přispěvatelé: | Health Research Board, HRA_POR/2012/123 |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Saliva Firmicutes Dental Plaque Dental Caries Gram-Positive Bacteria Polymerase Chain Reaction digestive system Microbiology Streptococcus mutans 03 medical and health sciences fluids and secretions 0302 clinical medicine stomatognathic system RNA Ribosomal 16S Prevotella Humans Medicine 030212 general & internal medicine Microbiome Child Children General Dentistry biology business.industry Microbiota Dentine Bacteroidetes 030206 dentistry biology.organism_classification medicine.disease lcsh:RK1-715 stomatognathic diseases Child Preschool lcsh:Dentistry Proteobacteria Early childhood caries business Research Article |
| Zdroj: | BMC Oral Health, Vol 19, Iss 1, Pp 1-14 (2019) BMC Oral Health |
| ISSN: | 1472-6831 |
| Popis: | peer-reviewed Background The main objectives of this study were to describe and compare the microbiota of 1) deep dentinal lesions of deciduous teeth of children affected with severe early childhood caries (S-ECC) and 2) the unstimulated saliva of these children and 3) the unstimulated saliva of caries-free children, and to compare microbiota compositional differences and diversity of taxa in these sampled sites. Methods Children with S-ECC and without S-ECC were recruited. The saliva of all children with and without S-ECC was sampled along with the deep dentinal microbiota from children affected by S-ECC. The salivary microbiota of children affected by S-ECC (n = 68) was compared to that of caries-free children (n = 70), by Illumina MiSeq sequencing of 16S rRNA amplicons. Finally, the caries microbiota of deep dentinal lesions of those children with S-ECC was investigated. Results Using two beta diversity metrics (Bray Curtis dissimilarity and UniFrac distance), the caries microbiota was found to be distinct from that of either of the saliva groups (caries-free & caries-active) when bacterial abundance was taken into account. However, when the comparison was made by measuring only presence and absence of bacterial taxa, all three microbiota types separated. While the alpha diversity of the caries microbiota was lowest, the diversity difference between the caries samples and saliva samples was statistically significant (p |
| Databáze: | OpenAIRE |
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