The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis
| Autor: | Di Sun, Kai Cui, Xiaomei Hu, Xiuren Zhang, Xingxing Yan, Zeyang Ma, Baoshuan Shang, Yanjun Li, Hisashi Koiwa, Niankui Li |
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| Rok vydání: | 2021 |
| Předmět: |
Small interfering RNA
Transgene Arabidopsis law.invention law Genetics Gene silencing Transgenes Epigenetics RNA Small Interfering Research Articles Multidisciplinary biology Arabidopsis Proteins Chemistry Plant Sciences fungi SciAdv r-articles RNA biology.organism_classification Cell biology RNA silencing Suppressor RNA Interference Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abf3898 |
| Popis: | The epigenetic factor FVE synchronizes SGS3-DRB4/DCL2/4 functions to promote transgenic siRNA production in the cytoplasm. Posttranscriptional gene silencing (PTGS) is a regulatory mechanism to suppress undesired transcripts. Here, we identified Flowering locus VE (FVE), a well-known epigenetic component, as a new player in cytoplasmic PTGS. Loss-of-function fve mutations substantially reduced the accumulation of transgene-derived small interfering RNAs (siRNAs). FVE interacts with suppressor of gene silencing 3 (SGS3), a master component in PTGS. FVE promotes SGS3 homodimerization that is essential for its function. FVE can bind to single-stranded RNA and double-stranded RNA (dsRNA) with moderate affinities, while its truncated form FVE-8 has a significantly increased binding affinity to dsRNA. These affinities affect the association and channeling of SGS3-RNA to downstream dsRNA binding protein 4 (DRB4)/Dicer-like protein 2/4 (DCL2/4) complexes. Hence, FVE, but not FVE-8, biochemically enhances the DRB4/DCL2/4 activity in vitro. We surmise that FVE promotes production of transgene-derived siRNAs through concertedly tuning SGS3-DRB4/DCL2/4 functions. Thus, this study revealed a noncanonical role of FVE in PTGS. |
| Databáze: | OpenAIRE |
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