Association between polymorphisms in HLA-A, HLA-B, HLA-DR, and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains: The first study in a Turkish population

Autor: Hamit Harun Dağ, Dogukan Ozbey, Hrisi Bahar Tokman, Harika Oyku Dinc, Nesrin Gareayaghi, Ihsan Tasci, Reyhan Gülergün, Suleyman Demiryas, Bekir Kocazeybek, Omer Uysal, Banu Tufan Kocak, Nuray Kepil, Huseyin Emre Dagdeviren, Mehmet Demirci, Erkan Yilmaz, Seher Akkus, Suat Saribas
Přispěvatelé: DİNÇ, HARİKA ÖYKÜ
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Turkey
Gastroenterology
Genotype
HLA-DQ beta-Chains
biology
The first study in a Turkish population.-
Infection
genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
cilt.82
ss.104288
2020 [Kocak B.
Saribas S.
Demiryas S.
Yilmaz E.
Uysal Ö.
Kepil N.
Demirci M.
Dınc H.
Akkus S.
Gülergün R.
et al.
-Association between polymorphisms in HLA-A
HLA-B
HLA-DR
and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains]

Middle Aged
HLA-A
Infectious Diseases
medicine.anatomical_structure
Female
Microbiology (medical)
Adult
medicine.medical_specialty
030106 microbiology
Human leukocyte antigen
Microbiology
HLA-DQ alpha-Chains
Helicobacter Infections
03 medical and health sciences
Young Adult
Bacterial Proteins
Stomach Neoplasms
Internal medicine
HLA-DQ Antigens
Genetics
Gastric mucosa
medicine
CagA
Humans
Genetic Predisposition to Disease
Allele
Molecular Biology
Ecology
Evolution
Behavior and Systematics

Aged
Antigens
Bacterial

Polymorphism
Genetic

HLA-A Antigens
Helicobacter pylori
Cancer
HLA-DR Antigens
biology.organism_classification
medicine.disease
digestive system diseases
030104 developmental biology
HLA-B Antigens
Case-Control Studies
Duodenal Ulcer
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 82
ISSN: 1567-7257
Popis: Colonization of the human gastric mucosa by H. pylori may cause peptic and duodenal ulcers (DUs), gastric lymphomas, and gastric cancers. The cagL gene is a component of cag T4SS and is involved in cagA translocation into host. An association between the risk of gastric cancer and the type of HLA class II (DR and/or DQ) was suggested in different populations. The aim of this study was to investigate, the clinical association of the cagL gene with host HLA alleles in H. pylori strains that were isolated from patients with gastric cancer, DU, and non-ulcer dyspepsia (NUD) and to determine the HLA allele that confers susceptibility or resistance for the risk of gastric cancer and DU development in Turkish patients. A total of 94 patients (44 gastric cancer and 50 DU patients; 58 male, 36 female; mean age, 49.6 years), and 86 individuals (50 NUD patients and 36 persons with normal gastrointestinal system [NGIS]; 30 male, 56 female; mean age, 47.3 years) were included as the patient and the control groups, respectively. CagA and cagL were determined by PCR method. DNA from peripheral blood samples was obtained by EZ-DNA extraction kit. For HLA SSO typing, LIFECODES SSO Typing kits (HLA-A, HLA-B HLA-C, HLA-DRB1 and HLA-DQA1/B1 kits) were used. The CagL/CagA positivity distribution in the groups were as follows: 42 (95.4%) gastric cancer, 46 (92%) DU and, 34 (68%) NUD and no NGIS cases. The HLA-DQA1*01 (OR: 3.82) allele was significantly different, suggesting that these individuals with H. pylori strains harbouring the CagL/CagA positivity are susceptible to the risk of gastric cancer and DU, and the HLA-DQA1*05 (OR, 0.318) allele was suggested as a protective allele for the risk of gastric cancer and DU using univariate analyses. HLA-DQA1*01 (OR, 2.21), HLA-DQB1*06 (OR, 2.67), sex (male, OR, 2.27), and CagL/CagA/(
Databáze: OpenAIRE
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