Association between polymorphisms in HLA-A, HLA-B, HLA-DR, and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains: The first study in a Turkish population
Autor: | Hamit Harun Dağ, Dogukan Ozbey, Hrisi Bahar Tokman, Harika Oyku Dinc, Nesrin Gareayaghi, Ihsan Tasci, Reyhan Gülergün, Suleyman Demiryas, Bekir Kocazeybek, Omer Uysal, Banu Tufan Kocak, Nuray Kepil, Huseyin Emre Dagdeviren, Mehmet Demirci, Erkan Yilmaz, Seher Akkus, Suat Saribas |
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Přispěvatelé: | DİNÇ, HARİKA ÖYKÜ |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Turkey Gastroenterology Genotype HLA-DQ beta-Chains biology The first study in a Turkish population.- Infection genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases cilt.82 ss.104288 2020 [Kocak B. Saribas S. Demiryas S. Yilmaz E. Uysal Ö. Kepil N. Demirci M. Dınc H. Akkus S. Gülergün R. et al. -Association between polymorphisms in HLA-A HLA-B HLA-DR and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains] Middle Aged HLA-A Infectious Diseases medicine.anatomical_structure Female Microbiology (medical) Adult medicine.medical_specialty 030106 microbiology Human leukocyte antigen Microbiology HLA-DQ alpha-Chains Helicobacter Infections 03 medical and health sciences Young Adult Bacterial Proteins Stomach Neoplasms Internal medicine HLA-DQ Antigens Genetics Gastric mucosa medicine CagA Humans Genetic Predisposition to Disease Allele Molecular Biology Ecology Evolution Behavior and Systematics Aged Antigens Bacterial Polymorphism Genetic HLA-A Antigens Helicobacter pylori Cancer HLA-DR Antigens biology.organism_classification medicine.disease digestive system diseases 030104 developmental biology HLA-B Antigens Case-Control Studies Duodenal Ulcer |
Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 82 |
ISSN: | 1567-7257 |
Popis: | Colonization of the human gastric mucosa by H. pylori may cause peptic and duodenal ulcers (DUs), gastric lymphomas, and gastric cancers. The cagL gene is a component of cag T4SS and is involved in cagA translocation into host. An association between the risk of gastric cancer and the type of HLA class II (DR and/or DQ) was suggested in different populations. The aim of this study was to investigate, the clinical association of the cagL gene with host HLA alleles in H. pylori strains that were isolated from patients with gastric cancer, DU, and non-ulcer dyspepsia (NUD) and to determine the HLA allele that confers susceptibility or resistance for the risk of gastric cancer and DU development in Turkish patients. A total of 94 patients (44 gastric cancer and 50 DU patients; 58 male, 36 female; mean age, 49.6 years), and 86 individuals (50 NUD patients and 36 persons with normal gastrointestinal system [NGIS]; 30 male, 56 female; mean age, 47.3 years) were included as the patient and the control groups, respectively. CagA and cagL were determined by PCR method. DNA from peripheral blood samples was obtained by EZ-DNA extraction kit. For HLA SSO typing, LIFECODES SSO Typing kits (HLA-A, HLA-B HLA-C, HLA-DRB1 and HLA-DQA1/B1 kits) were used. The CagL/CagA positivity distribution in the groups were as follows: 42 (95.4%) gastric cancer, 46 (92%) DU and, 34 (68%) NUD and no NGIS cases. The HLA-DQA1*01 (OR: 3.82) allele was significantly different, suggesting that these individuals with H. pylori strains harbouring the CagL/CagA positivity are susceptible to the risk of gastric cancer and DU, and the HLA-DQA1*05 (OR, 0.318) allele was suggested as a protective allele for the risk of gastric cancer and DU using univariate analyses. HLA-DQA1*01 (OR, 2.21), HLA-DQB1*06 (OR, 2.67), sex (male, OR, 2.27), and CagL/CagA/( |
Databáze: | OpenAIRE |
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