Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography

Autor:	Marian Hajduch, Eva Umlaufova, Vladimír Havlíček, Milos Petrik, Clemens Decristoforo, Andrea Palyzová, Zbynek Novy, Hubertus Haas, Dalibor Dolezal, Vladislav Raclavsky
Rok vydání:	2018
Předmět:	0301 basic medicine Siderophore Microbiological culture Low protein Iron lcsh:Medicine Siderophores Gallium Radioisotopes medicine.disease_cause Article Microbiology 03 medical and health sciences chemistry.chemical_compound Mice Positron Emission Tomography Computed Tomography medicine Animals Pseudomonas Infections Tissue Distribution lcsh:Science Mice Inbred BALB C Multidisciplinary Pyoverdine medicine.diagnostic_test biology Molecular Structure Chemistry Pseudomonas aeruginosa lcsh:R Pseudomonas Biological Transport biology.organism_classification In vitro Culture Media Rats 030104 developmental biology Positron emission tomography Positron-Emission Tomography lcsh:Q Radiopharmaceuticals Oligopeptides
Zdroj:	Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
ISSN:	2045-2322
Popis:	Pseudomonas aeruginosa is an increasingly prevalent opportunistic pathogen that causes a variety of life-threatening nosocomial infections. Novel strategies for the development of new antibacterial treatments as well as diagnostic tools are needed. One of the novel diagnostic strategies for the detection of infection could be the utilization of siderophores. Siderophores are low-molecular-weight chelators produced by microbes to scavenge essential iron. Replacing iron in siderophores by suitable radiometals, such as Ga-68 for positron emission tomography (PET) imaging, opens approaches for targeted imaging of infection. Here we report on pyoverdine PAO1 (PVD-PAO1), a siderophore produced by P. aeruginosa, labelled with Ga-68 for specific imaging of Pseudomonas infections. PVD-PAO1 was labelled with Ga-68 with high radiochemical purity. The resulting complex showed hydrophilic properties, low protein binding and high stability in human serum. In vitro uptake of 68Ga-PVD-PAO1 was highly dependent on the type of microbial culture. In normal mice 68Ga-PVD-PAO1 showed rapid pharmacokinetics with urinary excretion. PET imaging in infected animals displayed specific accumulation of 68Ga-PVD-PAO1 in infected tissues and better distribution than clinically used 18F-fluorodeoxyglucose (18F-FDG) and 68Ga-citrate. Ga-68 labelled pyoverdine PAO1 seems to be a promising agent for imaging of P. aeruginosa infections by means of PET.
Databáze:	OpenAIRE
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