Freeze-dried versus fresh platelet-rich plasma in acute wound healing of an animal model
| Autor: | Joyce M. Annichino-Bizzacchi, Stephany Cares Huber, Silmara Aparecida De Lima Montalvão, José Luiz Rosenberis Cunha Junior, Letícia Queiroz da Silva |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Embryology Pathology medicine.medical_specialty medicine.medical_treatment 0206 medical engineering Biomedical Engineering 02 engineering and technology 03 medical and health sciences Animal model In vivo medicine Animals Humans Rats Wistar 030304 developmental biology Cryopreservation Wound Healing 0303 health sciences Epidermis (botany) Platelet-Rich Plasma business.industry Growth factor Skin ulcer 020601 biomedical engineering Rats Acute wound Blood Preservation Platelet-rich plasma Acute Disease Wounds and Injuries medicine.symptom business Wound healing |
| Zdroj: | Regenerative Medicine. 14:525-534 |
| ISSN: | 1746-076X 1746-0751 |
| Popis: | Aim: To compare freeze-dried and fresh platelet-rich plasma (PRP) preparations, in a pre-clinical study. Materials & methods: 30 Wistar male rats were used to compare and characterize human PRP which was applied at the perilesional area in an acute wound model, evaluated by macroscopical and histological analysis. Results: Despite the increased growth factor concentration after the freeze-drying process, no change in the healing kinetics was observed in vivo. Nevertheless, a significant increased number of myofibroblasts was demonstrated in comparison with the fresh PRP group. We also demonstrated a significant increased percentage of blood vessels in comparison with controls in both the superficial and deep epidermis. Conclusion: These results encourage randomized clinical trials to evaluate the effectiveness of freeze-dried PRP for skin ulcer treatment. |
| Databáze: | OpenAIRE |
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