Group A streptococcal antigen exposed rat model to investigate neurobehavioral and cardiac complications associated with post‐streptococcal autoimmune sequelae
| Autor: | Adam S. Hamlin, Nicholas M. Andronicos, Ethan C Wilkinson, Catherine M Lobbe, Natkunam Ketheesan, Rukshan A M Rafeek, David J. McMillan, Kadaba S Sriprakash |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Medicine (General) Heart disease Streptococcus pyogenes Physiology medicine.disease_cause Group A Autoimmunity Marble burying R5-920 Streptococcal Infections medicine Animals Rats Wistar medicine.diagnostic_test business.industry Streptococcus animal model group A streptococcus Chorea paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections (PANDAS) Original Articles General Medicine rheumatic fever rheumatic heart disease medicine.disease Rats Rats Inbred Lew Lewis rats sydenham chorea (SC) Rheumatic fever Female Original Article medicine.symptom business Electrocardiography |
| Zdroj: | Animal Models and Experimental Medicine, Vol 4, Iss 2, Pp 151-161 (2021) Animal Models and Experimental Medicine |
| ISSN: | 2576-2095 |
| Popis: | Background The neuropsychiatric disorders due to post‐streptococcal autoimmune complications such as Sydenham's chorea (SC) are associated with acute rheumatic fever and rheumatic heart disease (ARF/RHD). An animal model that exhibits characteristics of both cardiac and neurobehavioral defects in ARF/RHD would be an important adjunct for future studies. Since age, gender, strain differences, and genotypes impact on the development of autoimmunity, we investigated the behavior of male and female Wistar and Lewis rat strains in two age cohorts (12 weeks) under normal husbandry conditions and following exposure to group A streptococcus (GAS). Methods Standard behavioral assessments were performed to determine the impairments in fine motor control (food manipulation test), gait and balance (beam walking test), and obsessive‐compulsive behavior (grooming and marble burying tests). Furthermore, electrocardiography, histology, and behavioral assessments were performed on male and female Lewis rats injected with GAS antigens. Results For control Lewis rats there were no significant age and gender dependent differences in marble burying, food manipulation, beam walking and grooming behaviors. In contrast significant age‐dependent differences were observed in Wistar rats in all the behavioral tests except for food manipulation. Therefore, Lewis rats were selected for further experiments to determine the effect of GAS. After exposure to GAS, Lewis rats demonstrated neurobehavioral abnormalities and cardiac pathology akin to SC and ARF/RHD, respectively. Conclusion We have characterised a new model that provides longitudinal stability of age‐dependent behavior, to simultaneously investigate both neurobehavioral and cardiac abnormalities associated with post‐streptococcal complications. |
| Databáze: | OpenAIRE |
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