OB protein binds specifically to the choroid plexus of mice and rats
| Autor: | Rene Devos, J Travernier, Louis A. Tartaglia, L A Campfield, J. Van der Heyden, Yves Guisez, Paul Burn, Geert Plaetinck, J G Richards |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Leptin
medicine.medical_specialty Recombinant Fusion Proteins Mice Obese Biology Tritium Iodine Radioisotopes Mice Cerebrospinal fluid In vivo Internal medicine medicine Animals Obesity Binding site Receptor Binding Sites Multidisciplinary Brain Proteins Alkaline Phosphatase Fusion protein Recombinant Proteins Rats Rats Zucker Mice Inbred C57BL Endocrinology Organ Specificity Choroid Plexus Autoradiography Alkaline phosphatase Choroid plexus Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:5668-5673 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.11.5668 |
| Popis: | Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|