Regulation of Neural Specification from Human Embryonic Stem Cells by BMP and FGF
| Autor: | Jason P. Weick, Matthew T. Pankratz, Jason R. Gerstner, Young Dong Yoo, Timothy M. LaVaute, Su-Chun Zhang |
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| Rok vydání: | 2009 |
| Předmět: |
animal structures
PAX6 Transcription Factor Cellular differentiation Fluorescent Antibody Technique Bone Morphogenetic Protein 4 Biology Bone morphogenetic protein Fibroblast growth factor Article Smad1 Protein Humans Paired Box Transcription Factors Phosphorylation Eye Proteins Cells Cultured Embryonic Stem Cells Homeodomain Proteins Neurons Cell Differentiation Cell Biology Immunohistochemistry Embryonic stem cell Molecular biology BMPR2 Cell biology Fibroblast Growth Factors Repressor Proteins Neuroepithelial cell Bone morphogenetic protein 4 Bone Morphogenetic Proteins embryonic structures Molecular Medicine Carrier Proteins Octamer Transcription Factor-3 Neural development Signal Transduction Developmental Biology |
| Zdroj: | Stem Cells. 27:1741-1749 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1002/stem.99 |
| Popis: | Inhibition of bone morphogenetic protein (BMP) signaling is required for vertebrate neural induction, and fibroblast growth factors (FGFs) may affect neural induction through phosphorylation at the linker region of Smad1, thus regulating BMP signaling. Here we show that human embryonic stem cells efficiently convert to neuroepithelial cells in the absence of BMP antagonists, or even when exposed to high concentrations of exogenous BMP4. Molecular and functional analyses revealed multiple levels of endogenous BMP signaling inhibition that may account for the efficient neural differentiation. Blocking FGF signaling inhibited neural induction, but did not alter the phosphorylation of the linker region of Smad1, suggesting that FGF enhances human neural specification independently of BMP signaling. Disclosure of potential conflicts of interest is found at the end of this article. |
| Databáze: | OpenAIRE |
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