Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes
| Autor: | Blake Kazaoka, Zulfiya Orynbayeva, David E. Stein, Richard Sensenig, Hanna Rapaport, Carleigh Charles, Ifat Cohen-Erez, Olga Melnikov |
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| Rok vydání: | 2020 |
| Předmět: |
Membrane potential
medicine.diagnostic_test Chemistry Biomedical Engineering Medicine (miscellaneous) Bioengineering Oxidative phosphorylation Development Mitochondrion law.invention Flow cytometry Mitochondria Drug Delivery Systems Pharmaceutical Preparations Confocal microscopy law Docking (molecular) Drug delivery Organelle medicine Biophysics Nanoparticles General Materials Science Peptides |
| Zdroj: | Nanomedicine (London, England). 15(30) |
| ISSN: | 1748-6963 |
| Popis: | Aims: The mechanistic study of the drug carrier–target interactions of mitochondria-unique nanoparticles composed of polypeptide–peptide complexes (mPoP-NPs). Materials & methods: The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles. Results: The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction. Conclusion: The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms. |
| Databáze: | OpenAIRE |
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