A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system
| Autor: | Gilbert Greub, Guy Prod'hom, Christian Durussel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Susceptibility testing medicine.drug_class Antibiotics Gram-Negative Bacteria/isolation & purification Microbial Sensitivity Tests Biology Gram-Positive Bacteria Microbiology Sensitivity and Specificity Anti-Bacterial Agents/pharmacology chemistry.chemical_compound Gram-Positive Bacteria/drug effects Gram-Negative Bacteria/classification Pellet Gram-Positive Bacteria/isolation & purification Drug Resistance Bacterial Gram-Negative Bacteria medicine Humans Blood culture Bacteriological Techniques Gram-Negative Bacteria/drug effects Gram-Positive Bacteria/classification medicine.diagnostic_test General Medicine biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents chemistry Ammonium chloride |
| Zdroj: | Journal of Medical Microbiology, vol. 62, no. Pt 5, pp. 773-777 |
| ISSN: | 1473-5644 |
| Popis: | An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99 % for Enterobacteriaceae and 74 % for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3 % for Enterobacteriaceae, and 0.7 and 0.1 % for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci. |
| Databáze: | OpenAIRE |
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