Imaging of Membrane Lipids in Single Cells by Imprint-Imaging Time-of-Flight Secondary Ion Mass Spectrometry
| Autor: | Lennart Carlsson, Håkan Nygren, Peter Sjövall, Per Malmberg, Jukka Lausmaa |
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| Rok vydání: | 2003 |
| Předmět: |
Diagnostic Imaging
Membranes Scanning electron microscope Chemistry Cells Membrane lipids Analytical chemistry Mass spectrometry Mass Spectrometry Analytical Chemistry Secondary ion mass spectrometry Membrane Lipids chemistry.chemical_compound Membrane Microscopy Microscopy Electron Scanning Biophysics Fluorescence microscope Humans Phosphocholine |
| Zdroj: | Analytical Chemistry. 75:3429-3434 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac0207675 |
| Popis: | A new method for identification and localization of organic molecules in biological samples is described. The method involves making an imprint of a biological sample on a silver (Ag) surface and subsequent analysis of the imprint by imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS). Using this method, detection of unfragmented, Ag cationized molecules at a spatial resolution of0.5 microm is possible. We have used the method to study the spatial distribution of phosphatidylcholine and cholesterol in blood cells adhering to a glass surface. The TOF-SIMS images show that cholesterol is preferentially located in the plasma membrane, whereas the phosphocholine shows highest concentration in the nuclear membrane. Scanning electron microscopy and fluorescence microscopy images show that the amount of transferred material during the imprinting process can be controlled by varying the imprinting pressure and pretreatment of the cell substrate prior to imprinting. |
| Databáze: | OpenAIRE |
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