Mesenchymal progenitor cells primed with pentosan polysulfate promote lumbar intervertebral disc regeneration in an ovine model of microdiscectomy
| Autor: | Ronil V. Chandra, Camilla A. Cohen, Tony Goldschlager, Graham Jenkin, Idrees Sher, Ronald Shimmon, Angela Vais, Tanya Badal, Kanika Jain, David Oehme, Andrew C.W. Zannettino, Chris D. Daly, Peter Ghosh |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Context (language use) Intervertebral Disc Degeneration Mesenchymal Stem Cell Transplantation 03 medical and health sciences 0302 clinical medicine Lumbar medicine Back pain Animals Regeneration Orthopedics and Sports Medicine Intervertebral Disc Cells Cultured Pentosan Sulfuric Polyester Sheep medicine.diagnostic_test business.industry Mesenchymal stem cell Lumbosacral Region Mesenchymal Stem Cells Magnetic resonance imaging Intervertebral disc Pentosan polysulfate Spinal column 030104 developmental biology medicine.anatomical_structure Surgery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery Diskectomy medicine.drug |
| Zdroj: | The Spine Journal. 18:491-506 |
| ISSN: | 1529-9430 |
| Popis: | Neural compression associated with lumbar disc herniation is usually managed surgically by microdiscectomy. However, 10%-20% of patients re-present with debilitating back pain, and approximately 15% require further surgery.Using an ovine model of microdiscectomy, the present study investigated the relative potential of pentosan polysulfate-primed mesenchymal progenitor cells (pMPCs) or MPC alone implanted into the lesion site to facilitate disc recovery.An ovine model of lumbar microdiscectomy was used to compare the relative outcomes of administering MPCs or pMPCs to the injury site postsurgery.At baseline 3T magnetic resonance imaging (MRI) of 18 adult ewes was undertaken followed by annular microdiscectomy at two lumbar disc levels. Sheep were randomized into three groups (n=6). The injured controls received no further treatment. Defects of the treated groups were implanted with a collagen sponge and MPC (5×10Both the MPC- and pMPC-injected groups exhibited less reduction in disc height (p.05) and lower Pfirrmann grades (p≤.001) compared with the untreated injury controls, but morphologic scores for the pMPC-injected discs were lower (p.05). The PG content of the AF injury site region (AF1) of pMPC discs was higher than MPC and injury control AF1 (p.05). At the AF1 and contralateral AF2 regions, the DNA content of pMPC discs was significantly lower than injured control discs and MPC-injected discs. Histologic and birefringent microscopy revealed increased structural organization and reduced degeneration in pMPC discs compared with MPC and the injured controls.In an ovine model 6 months after administration of pMPCs to the injury site disc PG content and matrix organization were improved relative to controls, suggesting pMPCs' potential as a postsurgical adjunct for limiting progression of disc degeneration after microdiscectomy. |
| Databáze: | OpenAIRE |
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