Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain
| Autor: | Ana Paula Konzen Riffel, Carolina Kolberg, Taina Scheid, J.A. de Souza, Maria do Carmo Quevedo Santos, W.A. Partata, A. Horst, Adriane Belló-Klein |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Physiology medicine.medical_treatment alpha-Tocopherol Total antioxidant capacity Antioxidantes Ascorbic Acid Peróxidos lipídicos medicine.disease_cause Biochemistry Antioxidants 0302 clinical medicine General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Research Articles Pain Measurement Superoxide anion generation lcsh:R5-920 General Neuroscience General Medicine Lipid hydroperoxides Antioxidant capacity Nociception medicine.anatomical_structure Spinal Cord Neuropathic pain Sciatic nerve lcsh:Medicine (General) Pain Threshold medicine.medical_specialty Immunology Biophysics Ocean Engineering 03 medical and health sciences Total thiol Internal medicine Estresse oxidativo medicine Animals Rats Wistar Peróxido de hidrogênio Vitamin C business.industry Vitamin E Superóxidos Nitric oxide Cell Biology Spinal cord Ascorbic acid Hydrogen peroxide Rats Disease Models Animal Oxidative Stress 030104 developmental biology Endocrinology lcsh:Biology (General) Sciatic Neuropathy business Biomarkers 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Brazilian Journal of Medical and Biological Research, Vol 51, Iss 4 (2018) Brazilian Journal of Medical and Biological Research v.51 n.4 2018 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 51, Issue: 4, Article number: e7097, Published: 01 MAR 2018 |
| Popis: | Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received injections of vitamins or vehicle (saline containing 1% Tween 80) for 3 or 10 days (n=6/each group). The vitamins prevented the reduction in total thiol content and the increase in superoxide-anion generation that were found in vehicle-treated CCI rats. While nitric-oxide metabolites increased in vehicle-treated CCI rats 3 days after surgery, these metabolites did not show significant changes in vitamin-treated CCI rats. In all rats, total antioxidant capacity and hydrogen-peroxide levels did not change significantly. Lipid hydroperoxides increased 25% only in vehicle-treated CCI rats. These changes may contribute to vit. C- and vit. E-induced antinociception, because scavenging reactive oxygen species seems to help normalize the spinal cord oxidative status altered by pain. |
| Databáze: | OpenAIRE |
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