Bioactive Compounds from the Aerial Parts of Brachystemma calycinum and Structural Revision of an Octacyclopeptide
| Autor: | Jun Zhao, Yong-Xian Cheng, Xi Li, Lili Zhou, Hong-Bin Xiao, Fan Fan Hou |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Collagen Type IV
Brachystemma Stereochemistry Pharmaceutical Science Caryophyllaceae Pharmacognosy Nitric Oxide Peptides Cyclic Collagen Type I Analytical Chemistry Nitric oxide Mice chemistry.chemical_compound Superoxides Drug Discovery Animals Amino Acid Sequence Viability assay Chemokine CCL2 Pharmacology chemistry.chemical_classification Reactive oxygen species Molecular Structure biology Interleukin-6 Superoxide Macrophages Organic Chemistry Biological activity biology.organism_classification Cyclic peptide Glucose Complementary and alternative medicine chemistry Mesangial Cells Molecular Medicine Reactive Oxygen Species Drugs Chinese Herbal |
| Zdroj: | Journal of Natural Products. 74:1392-1400 |
| ISSN: | 1520-6025 0163-3864 |
| Popis: | Four new cyclic peptides, brachystemins F-I (1-4), and 11 known compounds were isolated from the aerial parts of Brachystemma calycinim. The absolute configurations of compounds 1-4 were assigned using Marfey's method. The structure of compound 5 was revised from cyclo-(Pro(1)-Phe(2)-Leu(3)-Ala(4)-Thr(5)-Pro(6)-Ala(7)-Gly(8)) to cyclo-(Pro(1)-Pro(2)-Ala(3)-Gly(4)-Leu(5)-Ala(6)-Thr(7)-Phe(8)) with QTOF/MS and X-ray diffraction analysis. The N-containing compounds were assessed for their inhibitory effects on the secretion of monocyte chemokine ligand 2 (CCL-2), interleukin 6 (IL-6), and collagen IV against high-glucose-stimulated mesangial cells. Compound 5 was evaluated for its effects on collagen I, reactive oxygen species (ROS), superoxide anion (O(2)(center dot-)) production, and cell viability in mesangial cells, and on nitric oxide (NO) production in macrophage cells. |
| Databáze: | OpenAIRE |
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