Echinacoside and caffeoyl conjugates protect collagen from free radical-induced degradation: a potential use of Echinacea extracts in the prevention of skin photodamage
| Autor: | Pierluigi Mauri, Piergiorgio Pietta, Roberto Maffei Facino, Giancarlo Aldini, Marina Carini, L Saibene |
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| Rok vydání: | 1995 |
| Předmět: |
Xanthine Oxidase
Antioxidant Ultraviolet Rays medicine.medical_treatment Radical Pharmaceutical Science Xanthine Analytical Chemistry chemistry.chemical_compound Caffeic Acids Chlorogenic acid Drug Discovery medicine Caffeic acid Glycosides Xanthine oxidase Free-radical theory of aging Skin Pharmacology Cichoric acid Hydrolysis Organic Chemistry Free Radical Scavengers Complementary and alternative medicine chemistry Biochemistry Xanthines Echinacoside Molecular Medicine Collagen Reactive Oxygen Species |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0032-0943 |
| Popis: | The protective effect of caffeoyl derivatives (echinacoside, chlorogenic acid, chicoric acid, cynarine, and caffeic acid, typical constituents of Echinacea species) on the free radical-induced degradation of Type III collagen has been investigated. The macromolecule was exposed to a flux of oxygen radicals (superoxide anion and hydroxyl radical) generated by the xanthine/xanthine oxidase/Fe2+/EDTA system and its degradation assessed qualitatively by SDS-PAGE and quantitatively as the amount of soluble peptides (according to the 4-hydroxyproline method) released from native collagen after oxidative stress. The SDS-PAGE pattern of native collagen is markedly modified by free radical attack, with formation of a great number of peptide fragments with molecular masses below 97 kDa: in the presence of microM concentrations of echinacoside, there is a complete recovery of the native profile. Collagen degradation was, in fact, dose-dependently inhibited by all the compounds, with the following order of potency: echinacoside approximately chicoric acid > cynarine approximately caffeic acid > chlorogenic acid, with IC50 ranging from 15 to 90 microM. These results indicate that this representative class of polyphenols of Echinacea species protects collagen from free radical damage through a scavenging effect on reactive oxygen species and/or C-, N-, S-centered secondary radicals, and provide an indication for the topical use of extracts from Echinacea species for the prevention/treatment of photodamage of the skin by UVA/UVB radiation, in which oxidative stress plays a crucial role. |
| Databáze: | OpenAIRE |
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