P/Q-type and T-type voltage-gated calcium channels are involved in the contraction of mammary and brain blood vessels from hypertensive patients
| Autor: | Ole Skøtt, Frantz Rom Poulsen, Jane Stubbe, Anne D Thuesen, Lotte Rasmussen, Kristina S. Lyngsø, Lars Melholt Rasmussen, Pernille B. Lærkegaard Hansen, Christian Bonde Pedersen |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Contraction (grammar) hypertension Patch-Clamp Techniques T-type Physiology Cerebral arteries 030204 cardiovascular system & hematology Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Organ Culture Techniques Nifedipine Internal medicine medicine Humans Mammary Arteries L-type Aged Mibefradil Voltage-dependent calcium channel P/Q-type business.industry Calcium channel human arteries Cerebral Arteries Middle Aged Calcium Channel Blockers Immunohistochemistry penetrating arterioles Endocrinology Vasoconstriction Hypertension cardiovascular system Female Calcium Channels business Perfusion 030217 neurology & neurosurgery Myograph medicine.drug |
| Zdroj: | Thuesen, A D, Lyngsø, K S, Rasmussen, L, Stubbe, J, Skøtt, O, Rom Poulsen, F, Pedersen, C B, Rasmussen, L M & Hansen, P B L 2017, ' P/Q-type and T-type voltage-gated calcium channels are involved in the contraction of mammary and brain blood vessels from hypertensive patients ', Acta Physiologica, vol. 219, no. 3, pp. 642–653 . https://doi.org/10.1111/apha.12732 |
| ISSN: | 1748-1716 |
| DOI: | 10.1111/apha.12732 |
| Popis: | AIM: Calcium channel blockers are widely used in cardiovascular diseases. Besides L-type channels, T- and P/Q-type calcium channels are involved in the contraction of human renal blood vessels. It was hypothesized that T- and P/Q-type channels are involved in the contraction of human brain and mammary blood vessels.METHODS: Internal mammary arteries from bypass surgery patients and cerebral arterioles from patients with brain tumours with and without hypertension were tested in a myograph and perfusion set-up. PCR and immunohistochemistry were performed on isolated blood vessels.RESULTS: The P/Q-type antagonist ω-agatoxin IVA (10(-8) mol L(-1) ) and the T-type calcium blocker mibefradil (10(-7) mol L(-1) ) inhibited KCl depolarization-induced contraction in mammary arteries from hypertensive patients with no effect on blood vessels from normotensive patients. ω-Agatoxin IVA decreased contraction in cerebral arterioles from hypertensive patients. L-type blocker nifedipine abolished the contraction in mammary arteries. PCR analysis showed expression of P/Q-type (Cav 2.1), T-type (Cav 3.1 and Cav 3.2) and L-type (Cav 1.2) calcium channels in mammary and cerebral arteries. Immunohistochemical labelling of mammary and cerebral arteries revealed the presence of Cav 2.1 in endothelial and smooth muscle cells. Cav 3.1 was also detected in mammary arteries.CONCLUSION: P/Q- and T-type Cav are present in human internal mammary arteries and in cerebral penetrating arterioles. P/Q- and T-type calcium channels are involved in the contraction of mammary arteries from hypertensive patients but not from normotensive patients. Furthermore, in cerebral arterioles P/Q-type channels importance was restricted to hypertensive patients might lead to that T- and P/Q-type channels could be a new target in hypertensive patients. |
| Databáze: | OpenAIRE |
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