Marked Changes in Lamellar Granule and Trans-Golgi Network Structure Occur during Epidermal Keratinocyte Differentiation
| Autor: | Jiro Kishimoto, Haruyo Yamanishi, Akemi Ishida-Yamamoto, Toshihiko Hibino, Tsutomu Soma |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Keratinocytes Male 0301 basic medicine Stratum granulosum Human skin Dermatology Lamellar granule Biochemistry 03 medical and health sciences symbols.namesake Imaging Three-Dimensional 0302 clinical medicine medicine Humans Molecular Biology Aged integumentary system Chemistry Cell Differentiation Cell Biology Middle Aged Golgi apparatus 030104 developmental biology medicine.anatomical_structure Cytoplasm Transmission electron microscopy 030220 oncology & carcinogenesis Reticular connective tissue Microscopy Electron Scanning symbols Biophysics Female Epidermis trans-Golgi Network |
| Zdroj: | Journal of Investigative Dermatology. 139:352-359 |
| ISSN: | 0022-202X |
| Popis: | Epidermal lamellar granules transport various lipids, proteins, and protein inhibitors from the trans-Golgi network to the extracellular space, and play an important role in skin barrier formation. We elucidated the 3-dimensional structure of lamellar granules and the trans-Golgi network in normal human skin by focused ion beam scanning electron microscopy. Reconstructed focused ion beam scanning electron microscopy 3-dimensional images revealed that the overall lamellar granule structure changed from vesicular to reticular within the second layer of the stratum granulosum. Furthermore, the trans-Golgi network was well developed within this layer and spread through the cytoplasm with branched, tubular structures that connected to lamellar granules. Our study reveals the unique overall 3-dimensional structure of lamellar granules and the trans-Golgi network within the cells of the epidermis, and provides the basis for an understanding of the skin barrier formation. |
| Databáze: | OpenAIRE |
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