Supporting data on combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells
| Autor: | Varvara Chalmantzi, Theodore Fotsis, Marko Hyvönen, Angelos Papadopoulos, Dimitris Stellas, Carol Murphy, Marika Syrrou |
|---|---|
| Přispěvatelé: | Murphy, Carol [0000-0003-1353-8558], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Homeobox protein NANOG
Science (General) Computer applications to medicine. Medical informatics R858-859.7 Biology Regenerative medicine Transcriptome 03 medical and health sciences TGFβ Q1-390 0302 clinical medicine SOX2 medicine Data Article 030304 developmental biology 0303 health sciences mTeSR Multidisciplinary Effector Activin A medicine.disease Embryonic stem cell Cell biology embryonic structures Teratoma Human embryonic stem cells Signal transduction 030217 neurology & neurosurgery |
| Zdroj: | Data in Brief, Vol 35, Iss, Pp 106844-(2021) Data in Brief |
| ISSN: | 2352-3409 |
| Popis: | Human embryonic stem cells exhibit great potential as a therapeutic tool in regenerative medicine due to their self-renewal and trilineage differentiation capacity. Maintaining this unique cellular state has been shown to rely primarily on the Activin A / TGFβ signaling pathway. While most conventional culture media are supplemented with TGFβ, in the current study we utilize a modified version of the commercially available mTeSR1, substituting TGFβ for Activin A in order to preserve pluripotency. (1) Cells cultured in ActA-mTesR express pluripotency factors NANOG, OCT4 and SOX2 at comparable levels with cells cultured in TGFβ-mTeSR. (2) ActA-mTeSR cultured cells retain a physiological karyotype. (3) Cells in ActA-mTeSR maintain their trilineage differentiation capacity as shown in the teratoma formation assay. This system can be used to dissect the role of Activin A, downstream effectors and signaling cascades in human embryonic stem cell responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|