Intracellular localization of prenyltransferases of isoflavonoid phytoalexin biosynthesis in bean and soybean

Autor: Roland Welle, Hans Grisebach, David R. Biggs
Rok vydání: 1990
Předmět:
Zdroj: Planta. 181:244-248
ISSN: 1432-2048
0032-0935
DOI: 10.1007/bf02411546
Popis: The intracellular localization of prenyltransferases involved in the biosynthesis of the phytoalexins glyceollin in soybean (Glycine max L.) and phaseollin in French bean (Phaseolus vulgaris L.) has been investigated. By sucrose- and Percoll-gradient centrifugation of microsomes of an elicitor-challenged soybean cell culture, the membranes containing prenyltransferase were separated from the endoplasmic reticulum and shown to be lighter in density. In a continuous Percoll gradient the peak of prenyltransferase activity coincided with the peak of galactolipid synthesis, as determined by incorporation of uridine 5'-diphospho-[(14)C]galactose (UDP-[(14)C]galactose). Intact chloroplasts isolated from cupricchloride-treated bean leaves contained both prenyltransferase and UDP-galactose transferase activity. Both activities increased during chloroplast isolation. Fractionation of swollen chloroplasts on a discontinuous sucrose gradient showed prenyltransferase and UDP-galactose transferase activity in the envelope membrane subfraction. It is concluded that in both plants prenyltransferase is located in the envelope membrane of plastids.
Databáze: OpenAIRE
Externí odkaz: