Stability of Bio-Intact (1–84) Parathyroid Hormone ex Vivo in Serum and EDTA Plasma from Hemodialysis Patients
| Autor: | Joanna L. Wood, Edmund J. Lamb, Paul E. Stevens, Teresa K. Teal |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Parathyroid hormone Disorders of calcium metabolism Bone remodeling Serine Renal Dialysis Internal medicine medicine Humans Edetic Acid Aged Aged 80 and over Protease Chemistry Biochemistry (medical) Middle Aged Endocrinology Parathyroid Hormone Luminescent Measurements Female Hemodialysis Sample collection hormones hormone substitutes and hormone antagonists Ex vivo |
| Zdroj: | Clinical Chemistry. 50:1713-1714 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Measurement of circulating parathyroid hormone (PTH) is used in the differential diagnosis of disorders of calcium metabolism. Many samples come from patients with end-stage renal disease (ESRD), in whom PTH is used as an indicator of bone turnover (1). The instability of PTH in serum or plasma ex vivo has long been a concern. Use of EDTA-containing tubes for sample collection (2)(3)(4) reportedly improves stability, presumably as a result of protease inhibition (5). Several manufacturers recommend EDTA plasma for PTH measurement, but no information is available on the stability of PTH in ESRD samples measured with newer assays. The new full-length (also called “whole molecule” or “Bio-Intact”) PTH assays appear to recognize only forms containing the N-terminal serine. They are thought not to cross-react with truncated C-terminal fragments of PTH, in particular PTH(7–84), that increase in ESRD and cross-react in older assays. … |
| Databáze: | OpenAIRE |
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