A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism

Autor: Na Young Kim, Won Rak Son, Min Hoon Lee, Hong Seok Choi, Jun Young Choi, Young Jo Song, Chi Ho Yu, Dong Hyun Song, Gyeung Haeng Hur, Seong Tae Jeong, Sung Youl Hong, Young Kee Shin, Sungho Shin
Rok vydání: 2022
Předmět:
Zdroj: Applied Microbiology and Biotechnology. 106:1531-1542
ISSN: 1432-0614
0175-7598
DOI: 10.1007/s00253-022-11812-6
Popis: The potential use of biological agents has become a major public health concern worldwide. According to the CDC classification, Bacillus anthracis and Clostridium botulinum, the bacterial pathogens that cause anthrax and botulism, respectively, are considered to be the most dangerous potential biological agents. Currently, there is no licensed vaccine that is well suited for mass immunization in the event of an anthrax or botulism epidemic. In the present study, we developed a dual-expression system-based multipathogen DNA vaccine that encodes the PA-D4 gene of B. anthracis and the HCt gene of C. botulinum. When the multipathogen DNA vaccine was administered to mice and guinea pigs, high level antibody responses were elicited against both PA-D4 and HCt. Analysis of the serum IgG subtype implied a combined Th1/Th2 response to both antigens, but one that was Th2 skewed. In addition, immunization with the multipathogen DNA vaccine induced effective neutralizing antibody activity against both PA-D4 and HCt. Finally, the protection efficiency of the multipathogen DNA vaccine was determined by sequential challenge with 10 LD
Databáze: OpenAIRE
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