Comparative transcriptome analysis of rhizome nodes and internodes in Panax. japonicus var. major reveals candidate genes involved in the biosynthesis of triterpenoid saponins
| Autor: | Wenjun Zhu, Xiao-Hai Zhang, Tian Zuo, Ran Xu, Guang Wang, Shaopeng Zhang, Rufeng Wang, Ping Chen, Jingmao You |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Candidate gene Ginsenosides Panax Biology 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Triterpene Biosynthesis Botany Genetics Gene Plant Proteins 030304 developmental biology Plant stem chemistry.chemical_classification 0303 health sciences Glycosyltransferases Terpenoid Rhizome chemistry Metabolome Root Nodules Plant 010606 plant biology & botany |
| Zdroj: | Genomics. 112:1112-1119 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2019.06.025 |
| Popis: | The rhizome of P. japonicus var. major, one of the important herbs in Traditional Chinese medicine (TCM), has been used as tonic and hemostatic drugs in Tujia and Miao ethnic groups of China for thousand years. In this study, comparative metabolite and transcriptome analysis of rhizome nodes and internodes of wild P. japonicus var. major was performed to reveal their different roles in the biosynthesis of triterpene saponins. The results showed that the node was the crucial section for the synthesis of ginsenosides in the rhizome. The content of oleanane-type ginsenosides in the node was much higher than those in the internode. Most isoprenoid biosynthesis-related genes were highly expressed in the node. And, candidate UDP-glycosyltransferase (UGT) genes were also found to be differentially expressed between node and internode. Our study will provide a better understanding of the metabolism of ginsenosides in the rhizome of P. japonicus var. major. |
| Databáze: | OpenAIRE |
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