Vagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound
| Autor: | Ben W. Humrighouse, Anne M. Whitney, Tatiana C. A. Pinto, Edward R. B. Moore, John R. Barr, Patricia L. Shewmaker, Christopher A. Gulvik, Hercules Moura, Roger Karlsson, Jarrett Gartin, Lúcia M. Teixeira |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial Male food.ingredient Firmicutes Enterococcaceae medicine.disease_cause 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Vagococcus fluvialis food RNA Ribosomal 16S Vagococcus acidifermentans medicine Animals Humans Ecology Evolution Behavior and Systematics Phylogeny Base Composition Vagococcus biology Streptococcus Foot Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA rpoB biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Red Meat 030104 developmental biology Genes Bacterial Wounds and Injuries Cattle Vagococcus carniphilus |
| Zdroj: | International journal of systematic and evolutionary microbiology. 69(8) |
| ISSN: | 1466-5034 |
| Popis: | Two unusual catalase-negative, Gram-stain-positive, Vagococcus -like isolates that were referred to the CDC Streptococcus Laboratory for identification are described. Strain SS1994T was isolated from ground beef and strain SS1995T was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994T and SS1995T against Vagococcus type strain sequences supported their inclusion in the genus Vagococcus . Strain SS1994T showed high sequence similarity (>97.0 %) to the two most recently proposed species, Vagococcus martis (99.2 %) and Vagococcus teuberi (99.0 %) followed by Vagococcus penaei (98.8 %), strain SS1995T (98.6 %), Vagococcus carniphilus (98.0 %), Vagococcus acidifermentans (98.0 %) and Vagococcus fluvialis (97.9 %). The 16S rRNA gene sequence of strain SS1995T was most similar to V. penaei (99.1 %), followed by SS1994T (98.6 %), V. martis (98.4 %), V. teuberi (98.1 %), V. acidifermentans (97.8 %), and both V. carniphilus and V. fluvialis (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, rpoA, rpoB, pheS and groL genes, and comparative core and whole genome sequence analyses revealed that strains SS1994T and SS1995T were two novel Vagococcus species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|