The effect of mesenchymal stem cells on chemotaxis of osteoclast precursor cells
| Autor: | Tetsuya Awada, Nanae Oki, Kotaro Tanimoto, Yuji Tsuka, Kazuyo Ando, Kengo Nakajima, Keisuke Sumi, Takaharu Abe, Ryo Kunimatsu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemokine Bone Regeneration Receptors CCR2 Cellular differentiation Gene Expression Osteoclasts Real-Time Polymerase Chain Reaction Mice 03 medical and health sciences 0302 clinical medicine Osteoclast medicine Animals Bone regeneration General Dentistry Chemokine CCL2 Mice Inbred BALB C Cell chemotaxis biology Chemistry Chemotaxis Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells hemic and immune systems 030206 dentistry Coculture Techniques Cell biology RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure biology.protein RNA |
| Zdroj: | Journal of Oral Science. 60:221-225 |
| ISSN: | 1880-4926 1343-4934 |
| DOI: | 10.2334/josnusd.17-0187 |
| Popis: | Regeneration of tissue, including bone, using mesenchymal stem cells (MSCs) has been progressing rapidly. Regeneration of bone requires the presence of an appropriate environment and efficient chemotaxis of cells to the target site. Differentiation of MSCs into mesenchymal cells has received considerable attention, but the effect of MSCs on chemotaxis is not well understood. In this study, we investigated the effect of MSCs on chemotaxis of RAW264 cells via C-C motif chemokine ligand 2 (CCL2). Balb/c mouse bone marrow-derived MSCs and RAW264 cells, which are osteoclast precursor cells, were co-cultured without cell contact. The gene expression of CCL2 in MSCs and CC-chemokine receptor 2 (CCR2) in RAW264 cells was determined using quantitative real-time PCR. Analysis of RAW264 cell chemotaxis was performed using the Boyden chamber assay. mRNAs for CCL2 and CCR2 were significantly upregulated upon co-culture in comparison to culture of either cell type alone, and the number of chemotactic RAW264 cells was significantly increased by co-culture. MSCs enhanced the chemotaxis of RAW264 cells, possibly via CCL2-CCR2 interaction, suggesting the potential utility of MSCs for tissue regeneration. |
| Databáze: | OpenAIRE |
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