Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens
| Autor: | Carlos A. Salgueiro, Pilar C. Portela, Marta Bruix, Leonor Morgado, David Pantoja-Uceda, Liliana R. Teixeira |
|---|---|
| Přispěvatelé: | Fundação para a Ciência e a Tecnologia (Portugal), Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2019 |
| Předmět: |
Cytochrome
030303 biophysics Cytochrome: PccH Heme Biochemistry Redox 03 medical and health sciences chemistry.chemical_compound Electron transfer Microbial electrosynthesis Structural Biology Nuclear Magnetic Resonance Biomolecular Geobacter sulfurreducens 030304 developmental biology 0303 health sciences biology Biofilm Metabolism biology.organism_classification NMR chemistry PccH [Cytochrome] biology.protein Cytochromes Geobacter Oxidation-Reduction |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1874-270X 1874-2718 |
| DOI: | 10.1007/s12104-019-09899-6 |
| Popis: | 6 pags., 2 figs., 1 tab. Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (H, C and N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners. This work was supported by the following Fundação para a Ciência e a Tecnologia (FCT-MCTES) Grants: PTDC/BIA-BQM/31981/2017 (to CAS) and scholarship Grant No. PD/BD/114445/2016 (to LRT) through Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012, UID/FIS/00068/2013 (CEFITEC). This work was also supported by Unidade de Ciências Biomoleculares Aplicadas-UCIBIO which is fnanced by national funds from FCT/MEC (UID/Multi/04378/2013) and co-fnanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We acknowledge the use of the “Manuel Rico” NMR laboratory, LMR (CSIC), a Spanish large-scale national NMR facility ICTS R-LRB. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink