Hrb27C, Sqd and Otu cooperatively regulategurkenRNA localization and mediate nurse cell chromosome dispersion inDrosophilaoogenesis
| Autor: | Trudi Schüpbach, K. Nicole Clouse, Jennifer S. Goodrich |
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| Rok vydání: | 2004 |
| Předmět: |
RNA localization
RNA-binding protein Heterogeneous ribonucleoprotein particle Models Biological Chromosomes Heterogeneous-Nuclear Ribonucleoproteins Animals Genetically Modified Oogenesis Two-Hybrid System Techniques Translational regulation Animals Drosophila Proteins Molecular Biology Body Patterning Genetics Polytene chromosome biology Ovary RNA-Binding Proteins Transforming Growth Factor alpha biology.organism_classification Drosophila melanogaster Phenotype Mutation RNA splicing Oocytes RNA Female Drosophila Protein Developmental Biology |
| Zdroj: | Development. 131:1949-1958 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.01078 |
| Popis: | Heterogeneous nuclear ribonucleoproteins, hnRNPs, are RNA-binding proteins that play crucial roles in controlling gene expression. In Drosophilaoogenesis, the hnRNP Squid (Sqd) functions in the localization and translational regulation of gurken (grk) mRNA. We show that Sqd interacts with Hrb27C, an hnRNP previously implicated in splicing. Like sqd, hrb27C mutants lay eggs with dorsoventral defects and Hrb27C can directly bind to grk RNA. Our data demonstrate a novel role for Hrb27C in promoting grk localization. We also observe a direct physical interaction between Hrb27C and Ovarian tumor (Otu), a cytoplasmic protein implicated in RNA localization. We find that some otu alleles produce dorsalized eggs and it appears that Otu cooperates with Hrb27C and Sqd in the oocyte to mediate proper grklocalization. All three mutants share another phenotype, persistent polytene nurse cell chromosomes. Our analyses support dual cooperative roles for Sqd,Hrb27C and Otu during Drosophila oogenesis. |
| Databáze: | OpenAIRE |
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