Myelinated mouse nerves studied by X-ray phase contrast zoom tomography
| Autor: | Martin Krenkel, Wiebke Möbius, Tim Salditt, Matthias Bartels, Peter Cloetens |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Osmium Tetroxide
media_common.quotation_subject Mice Myelin chemistry.chemical_compound Imaging Three-Dimensional Nuclear magnetic resonance Structural Biology medicine Animals Contrast (vision) Microscopy Phase-Contrast Saphenous Vein Axon Myelin Sheath media_common Node of Ranvier Staining and Labeling Optic Nerve Anatomy Sciatic Nerve Axons Mice Inbred C57BL medicine.anatomical_structure nervous system Osmium tetroxide chemistry Optic nerve Schwann Cells Sciatic nerve Tomography Tomography X-Ray Computed |
| Zdroj: | Journal of Structural Biology. 192:561-568 |
| ISSN: | 1047-8477 |
| DOI: | 10.1016/j.jsb.2015.11.001 |
| Popis: | We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic internodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect