Allyl isothiocyanate attenuates oxidative stress and inflammation by modulating Nrf2/HO-1 and NF-kappa b pathways in traumatic brain injury in mice
| Autor: | Berrak Caglayan, Fusun Erten, Serdar Altunay, Mehmet Yalçın Günal, Cemal Orhan, Mehmet Tuzcu, Vijaya Juturu, Kazim Sahin, Burak Yulug, Ertugrul Kilic, Arman Dalay |
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| Přispěvatelé: | Caglayan, Berrak Istanbul Medipol Univ, Dept Med Biol, Int Sch Med, Istanbul, Turkey, Kilic, Ertugrul, Dalay, Arman, Altunay, Serdar Istanbul Medipol Univ, Dept Physiol, Istanbul, Turkey, Caglayan, Berrak, Altunay, Serdar, Gunal, Mehmet Yalcin, Yulug, Burak Istanbul Medipol Univ, Regenerat & Restorat Med Res Ctr, Istanbul, Turkey, Tuzcu, Mehmet, Erten, Fusun Firat Univ, Div Biol, Fac Sci, Elazig, Turkey, Orhan, Cemal, Sahin, Kazim Firat Univ, Sch Vet Med, Dept Anim Nutr, Elazig, Turkey, Yulug, Burak Istanbul Medipol Univ, Sch Med, Dept Neurol, Istanbul, Turkey, Juturu, Vijaya OmniAct Hlth Technol Inc, Res & Dev, Morristown, NJ USA, Orhan, Cemal -- 0000-0003-4138-7689, gunal, mehmet yalcin -- 0000-0001-7702-2441, Kilic, Ertugrul -- 0000-0001-6494-8923, Burak -- 0000-0002-9704-6173, Erten, Fusun -- 0000-0003-1657-7253 |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Antioxidant Traumatic Brain Injury medicine.medical_treatment Interleukin-1beta Pharmacology medicine.disease_cause Antioxidants chemistry.chemical_compound Mice 0302 clinical medicine Isothiocyanates Brain Injuries Traumatic Medicine chemistry.chemical_classification Glial fibrillary acidic protein biology General Medicine Allyl isothiocyanate Intercellular Adhesion Molecule-1 030220 oncology & carcinogenesis Cytokines medicine.symptom NF-Kappa B Signal Transduction NF-E2-Related Factor 2 Inflammation Nrf2 Proinflammatory cytokine 03 medical and health sciences Glial Fibrillary Acidic Protein Genetics Animals Molecular Biology Reactive oxygen species business.industry Interleukin-6 Membrane Proteins NFKB1 Mice Inbred C57BL Disease Models Animal Oxidative Stress 030104 developmental biology chemistry Brain Injuries biology.protein business Reactive Oxygen Species Oxidative stress Heme Oxygenase-1 Allyl Isothiocyanate |
| Popis: | WOS: 000462022300026 PubMed ID: 30406889 Traumatic brain injury (TBI) is the leading cause of mortality and morbidity in young adults and children in the industrialized countries; however, there are presently no FDA approved therapies. TBI results in oxidative stress due to the overproduction of reactive oxygen species and overwhelming of the endogenous antioxidant mechanisms. Recently, it has been reported that antioxidants including phytochemicals have a protective role against oxidative damage and inflammation after TBI. To analyze the effects of a naturally occurring antioxidant molecule, allyl isothiocyanate (AITC), on the nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa B (NF-kappa B) signaling pathways in TBI, a cryogenic injury model was induced in mice. Here, we showed that AITC administered immediately after the injury significantly decreased infarct volume and blood-brain barrier (BBB) permeability. Protein levels of proinflammatory cytokines interleukin-1 beta (IL1 beta) and interleukin-6 (IL6), glial fibrillary acidic protein (GFAP) and NF-kappa B were decreased, while Nrf2, growth-associated protein 43 (GAP43) and neural cell adhesion molecule levels were increased with AITC when compared with vehicle control. Our results demonstrated that the antioxidant molecule AITC, when applied immediately after TBI, provided beneficial effects on inflammatory processes while improving infarct volume and BBB permeability. Increased levels of plasticity markers, as well as an antioxidant gene regulator, Nrf2, by AITC, suggest that future studies are warranted to assess the protective activities of dietary or medicinal AITC in clinical studies. OmniActive Health Technologies Inc.; Turkish Academy of Sciences This study was supported by OmniActive Health Technologies Inc. (NJ, USA). This work was also supported in part by the Turkish Academy of Sciences. |
| Databáze: | OpenAIRE |
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