Analysis of Basement Membrane Structure and Inflammation During the Development of Esophageal Squamous Cell Carcinoma in the Chinese Chaoshan High Risk Region

Autor:	Dongping Tian, Hai-Hua Huang, Xian-Ying Wu, Ruiming Zheng, Min Su, Qiao-Shan Li, Guo-Hong Zhang
Rok vydání:	2008
Předmět:	China Cancer Research Pathology medicine.medical_specialty Integrin beta Chains Esophageal Neoplasms Blotting Western H&E stain Biology Basement Membrane Risk Factors Laminin medicine Carcinoma Humans Extracellular Signal-Regulated MAP Kinases Cell Proliferation Inflammation Basement membrane Reverse Transcriptase Polymerase Chain Reaction Carcinoma in situ General Medicine Hyperplasia medicine.disease Immunohistochemistry Epithelium Cell Transformation Neoplastic Ki-67 Antigen medicine.anatomical_structure Oncology Tissue Array Analysis Dysplasia Carcinoma Squamous Cell biology.protein Signal Transduction
Zdroj:	Cancer Investigation. 26:296-305
ISSN:	1532-4192 0735-7907
Popis:	To investigate relationships between basement membrane structure, inflammation, beta1 integrin expression, activation of ERK/MAPK signaling pathways, and cell proliferation in esophageal mucosa at various stages during the evolution of esophageal squamous cell carcinoma.Three tissue arrays were made of 228 tissue cores from 428 surgically-resected specimens. The arrays included 26 samples of normal epithelium, 28 with hyperplasia, 18 with dysplasia, 27 with carcinoma in situ and 129 with invasive carcinoma. In addition, 21 cases of hyperplasia, 13 cases of dysplasia and 13 case of carcinoma in situ were obtained by manual microdissection of unfixed frozen tissue. Hematoxylin and eosin stained sections were used to evaluate the epithelium and inflammation. The periodic acid-Schiff stain and an immunohistochemical stain for laminin were used to examine the structure of basement membranes. The expression of beta1 integrin, p-ERK, and Ki67 were evaluated by quantitative immunohistochemistry. RT-PCR and Western blots were also used to detect expression of beta1 integrin.Quantitative scales were developed to classify basement membrane structure and inflammation. Basement membrane alterations correlated with the degree of epithelial change (chi2 = 501.9, p0.01) and with the degree of lymphocytic infiltration in the lamina propria and epithelium (chi2 = 273.4, p0.01). There was a significant relationship between the extent of basement membrane alteration and the expression of beta1 integrin, p-ERK, and Ki67.The correlations suggest that there is a direct relationship between basement membrane structure and the development of esophageal squamous cell carcinoma.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::497775eed9018f30387f7ed43de98077 https://doi.org/10.1080/07357900701683901 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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