Carrageenan and Delayed Hypersensitivity II. Activation of Hageman Factor by Carrageenan and Its Possible Significance
| Autor: | Howard J. Schwartz, Robert W. Kellermeyer |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Factor XII
Guinea Pigs Vascular permeability Kinins Kinin Pharmacology Carrageenan Permeability General Biochemistry Genetics and Molecular Biology Complement system chemistry.chemical_compound Coagulation Biochemistry chemistry In vivo Delayed hypersensitivity Methods Animals Blood Vessels Humans Hypersensitivity Delayed Blood Coagulation Tests Blood Coagulation |
| Zdroj: | Experimental Biology and Medicine. 132:1021-1024 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-132-34358 |
| Popis: | Carrageenan is a high molecular weight, long-chain polymer of sulfated galactose units that can be extracted from marine plants. Carrageenan has recently been shown to suppress the delayed hypersensitivity reactions of sensitized guinea pigs (1), inhibit the complement system (2, 3), damage macrophages in vitro (4) and in vivo (5), and induce kinin formation in rat plasma (6).In this paper we show that carrageenan activates Hageman factor (factor XII) in human plasma, and thus promotes blood coagulation, induces vascular permeability, and liberates kinin (7). At higher concentrations it also acts as an anticoagulant, as reported previously (8).Materials and Methods. Carrageenan was kindly supplied by Marine Colloids, Inc., Springfield, New Jersey as Seakem 21. The powder was dissolved in hot saline prior to use, using a double boiler technique.Human platelet-deficient plasma was prepared in silicone coated plastic equipment. Venous blood was drawn into 30-ml plastic syringes, mixed with 0.1 vol of 0.13 M ... |
| Databáze: | OpenAIRE |
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