Mycobacterium aquaticum sp. nov., a rapidly growing species isolated from haemodialysis water
| Autor: | Emanuele Borroni, Alberto Trovato, Roberta Mannino, Parvin Haidarieh, Enrico Tortoli, Mohamad Hashemzadeh, Abdolrazagh Hashemi Shahraki, Sara Droz, Alessandro Mariottini, Daniela Maria Cirillo, Mehdi Mirsaeidi |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial Sequence analysis Iran 010603 evolutionary biology 01 natural sciences Microbiology Mycobacterium 03 medical and health sciences Mycobacterium brisbanense Renal Dialysis RNA Ribosomal 16S Humans Ecology Evolution Behavior and Systematics Phylogeny Base Composition biology General Medicine Sequence Analysis DNA Ribosomal RNA rpoB biology.organism_classification 16S ribosomal RNA Housekeeping gene Bacterial Typing Techniques 030104 developmental biology Mycolic Acids Genes Bacterial Mycobacterium fortuitum Water Microbiology |
| DOI: | 10.7892/boris.138217 |
| Popis: | The characterization of five Iranian isolates, four from hospital haemodialysis water and one from the sputum of a patient, led to the detection of a novel mycobacterium species. The strains were characterized by mucoid colonies developing in 3–5 days at temperatures ranging from 25 to 37 °C. The biochemical test pattern was unremarkable while the HPLC profile of mycolic acids resembled that of Mycobacterium fortuitum . The sequences of three major housekeeping genes (16S rRNA, hsp65 and rpoB) were unique and differed from those of any other mycobacterium. Mycobacterium brisbanense , which is the species that shared the highest 16S rRNA gene sequence similarity (99.03 %), was distinct, as shown by the average nucleotide identity and by the genome to genome distance values (91.05 and 43.10 %, respectively). The strains are thus considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium aquaticum sp. nov. is proposed. The type strain is RW6T (=DSM 104277T=CIP111198T). |
| Databáze: | OpenAIRE |
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