Differentiation of Streptomyces spp. which cause potato scab disease on the basis of partial rpoB gene sequences
| Autor: | Keun-Hwa Lee, Bum Joon Kim, Ho Suk Mun, Eun-Ju Oh, Young-Hwan Koh, Hyun-Ju Kim, Jin-Won Hyun, Chang-Jin Kim |
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| Rok vydání: | 2007 |
| Předmět: |
Phylogenetic tree
biology Molecular Sequence Data Nucleic acid sequence DNA-Directed RNA Polymerases rpoB biology.organism_classification 16S ribosomal RNA DNA Ribosomal Applied Microbiology and Biotechnology Microbiology Streptomyces Plant disease Phylogenetics RNA Ribosomal 16S Kitasatospora Phylogeny Ecology Evolution Behavior and Systematics Plant Diseases Solanum tuberosum |
| Zdroj: | Systematic and Applied Microbiology. 30:401-407 |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/j.syapm.2007.01.003 |
| Popis: | In a previous study, we found that the phylogenetic analysis of partial rpoB sequences can be used effectively to phylogenetically differentiate Streptomyces spp. [B.J. Kim, C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, Y.H. Kook, Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase beta-subunit gene (rpoB) sequences, Int. J. Syst. Evol. Microbiol. 54 (2004) 593-598]. In the present study, we analyzed the partial rpoB gene sequences of 19 reference Streptomyces strains associated with potato scab. Furthermore, to empirically confirm the usefulness of rpoB gene analysis for the phylogenetic differentiation of Streptomyces spp., we applied the proposed system to 27 potato scab isolates obtained from the Korean provinces of Jeju-do and Kangwon-do. Phylogenetic relationships among these isolates using the devised rpoB gene-based methods were generally similar to those reported for 16S rRNA gene-based analysis. Isolates from potato scab lesion in Korea were also clearly differentiated into their phylogenetic groups by this method. In addition, the deduced RpoB amino acid sequences were also found to be useful for differentiating these strains. Our data demonstrate that the rpoB gene-based method can be used as a means of complementing other genetic methods such as 16S rRNA gene analysis or DNA-DNA hybridization to phylogenetically differentiate potato scab related Streptomyces spp. |
| Databáze: | OpenAIRE |
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