The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants
| Autor: | DongHyuk Lee, Gitta Coaker, Daolong Dou, Katayoon Dehesh, Savithramma P. Dinesh-Kumar, Jianmin Zhou, Shisong Ma, Miaomiao Ma, Xiangxiu Liang, Mark S. Lemos, Yi-Hsuan Chiang, Neeraj K. Lal, Meixiang Zhang, Tania Y. Toruño, Jun Liu, Yi-Ju Lu, Brad Day |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mutant Regulator Arabidopsis Pseudomonas syringae Protein Serine-Threonine Kinases Microbiology Article 03 medical and health sciences Gene Expression Regulation Plant Virology Extracellular Plant Immunity Phosphorylation Plant Diseases NADPH oxidase biology Kinase Arabidopsis Proteins fungi NADPH Oxidases biology.organism_classification Anti-Bacterial Agents Cell biology 030104 developmental biology biology.protein Parasitology Reactive Oxygen Species Microtubule-Associated Proteins Flagellin |
| Zdroj: | Cell hostmicrobe. 24(3) |
| ISSN: | 1934-6069 |
| Popis: | Microbial patterns are recognized by cell-surface receptors to initiate pattern-triggered immunity (PTI) in plants. Receptor-like cytoplasmic kinases (RLCKs), such as BIK1, and calcium-dependent protein kinases (CPKs) are engaged during PTI to activate the NADPH oxidase RBOHD for reactive oxygen species (ROS) production. It is unknown whether protein kinases besides CPKs and RLCKs participate in RBOHD regulation. We screened mutants in all ten Arabidopsis MAP4 kinases (MAP4Ks) and identified the conserved MAP4K SIK1 as a positive regulator of PTI. sik1 mutants were compromised in their ability to elicit the ROS burst in response to microbial features and exhibited compromised PTI to bacterial infection. SIK1 directly interacts with, phosphorylates, and stabilizes BIK1 in a kinase activity-dependent manner. Furthermore, SIK1 directly interacts with and phosphorylates RBOHD upon flagellin perception. Thus, SIK1 positively regulates immunity by stabilizing BIK1 and activating RBOHD to promote the extracellular ROS burst. |
| Databáze: | OpenAIRE |
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