Keratocyte density and size in conscious humans by digital image analysis of confocal images
| Autor: | P N Dilly, M Kerr Muir, John Marshall, J I Prydal, Melanie C. Corbett, F Franc |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Consciousness Confocal Cell Count Image processing Biology law.invention Cornea Digital image Confocal microscopy law Microscopy Image Processing Computer-Assisted medicine Humans Cell Size Microscopy Confocal Resolution (electron density) Epithelium Corneal Anatomy Epithelium Ophthalmology medicine.anatomical_structure Female Biomedical engineering |
| Zdroj: | Eye. 12:337-342 |
| ISSN: | 1476-5454 0950-222X |
| DOI: | 10.1038/eye.1998.82 |
| Popis: | PURPOSE: Confocal microscopy can give images of high magnification and resolution in undisturbed living tissue. It provides new information about the cellular structure of the cornea. Our aim was to measure the density, size and distribution of keratocytes. METHODS: Healthy cornea in four subjects was examined using tandem scanning confocal microscopy. Methods for digital analysis of images were developed. RESULTS: Keratocyte density in confocal cross-sections was greatest immediately under Bowman's membrane (maximum 800 cells/mm2) and decreased sharply towards posterior cornea (minimum 65 cells/mm2). Cross-sectional cell size ranged from 78 to 211 microns2, but did not correlate with depth in the tissue. CONCLUSIONS: Results are consistent with those of earlier work using histological and biochemical techniques in isolated tissue. The methods we have developed enable studies of ongoing processes in conscious humans and can be used to examine diseased tissue as well as the response to injury. |
| Databáze: | OpenAIRE |
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