Low expression of miR-20b-5p indicates favorable prognosis in laryngeal squamous cell carcinoma, especially in patients with non-infiltrated regional lymph nodes
| Autor: | Ioannis Giotakis, Aliki Liakea, Theodwros-Leonidas Pantazis, Aris I. Giotakis, Manousos Konstantoulakis, Evangelos P. Misiakos, Sotirios Karamagkiolas |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Oncology medicine.medical_specialty Gene Expression Favorable prognosis Internal medicine Biomarkers Tumor medicine Humans In patient Laryngeal Neoplasms Aged Aged 80 and over Squamous Cell Carcinoma of Head and Neck business.industry Head and neck cancer Middle Aged Prognosis medicine.disease Laryngeal squamous cell carcinoma Reverse transcriptase Gene Expression Regulation Neoplastic MicroRNAs Real-time polymerase chain reaction Otorhinolaryngology Lymphatic Metastasis Carcinoma Squamous Cell Biomarker (medicine) Female Lymph Nodes Lymph business |
| Zdroj: | American Journal of Otolaryngology. 41:102563 |
| ISSN: | 0196-0709 |
| DOI: | 10.1016/j.amjoto.2020.102563 |
| Popis: | Tumor recurrence and distant metastasis are very common in laryngeal squamous cell carcinoma (LSCC). In this study, we examined the potential prognostic value of microRNA-20b-5p (miR-20b-5p), a component of the tumor-related miR-106a/363 cluster.Total RNA was purified from 105 tissue specimens resected from patients having undergone surgical treatment for primary LSCC. After in vitro polyadenylation and reverse transcription, a sensitive real-time quantitative polymerase chain reaction (qPCR) methodology was applied for the relative quantification of miR-20b-5p levels. Then, we proceeded with biostatistical analysis, seeking to assess the prognostic value of miR-20b-5p expression in LSCC.miR-20b-5p positivity constitutes a predictor of inferior DFS and OS in LSCC (P 0.001 and P = 0.002, respectively). The significant prognostic value of miR-20b-5p expression status seems to be independent of tumor size, histological grade, and TNM stage, as revealed by the multivariate bootstrap Cox regression analysis. Kaplan-Meier survival analysis showed also that miR-20b-5p expression status can stratify LSCC patients with non-infiltrated regional lymph nodes (N0) into two subgroups with distinct prognosis (P = 0.004 and P = 0.004, respectively).The miR-20b-5p expression status is a promising molecular tissue biomarker in LSCC, with an independent prognostic value, and thus merits further validation in larger cohorts of patients. |
| Databáze: | OpenAIRE |
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