Assembling a single-molecule view on nucleosome dynamics

Autor:	Rifka Vlijm
Přispěvatelé:	Dekker, C., Dekker, Cees, Molecular Biophysics
Jazyk:	angličtina
Rok vydání:	2014
Předmět:	nucleosome torque tetrasome DNA single molecule AFM histone-chaperone magnetic tweezers plectoneme dynamics histone-chapreone
Zdroj:	None University of Groningen
DOI:	10.4233/uuid:87c56120-cbf8-4f80-8a8b-ca66cd90aa5f
Popis:	The main focus of this thesis is a better understanding of the basic compaction mechanism of our DNA using multiple single-molecule techniques. The stretched-out length of our DNA is enormous compared with the dimensions of a cell. To make DNA fit within a cell it is systematically wrapped around proteins (histone octamers), forming nucleosomes. Besides compacting, nucleosomes also decrease the necessary accessibility of DNA for important processes like transcription and replication. The dynamical interplay between DNA and proteins ensures the right balance between compact and accessible DNA, but is not yet fully understood. Therefore, we study the process of nucleosome assembly as well as the dynamics of nucleosomes at the single-molecule level. Furthermore, we investigate proteins that probe the conformation of the DNA helical structure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::496981987e5ffa4bbd31b2e643508801 https://doi.org/10.4233/uuid:87c56120-cbf8-4f80-8a8b-ca66cd90aa5f Zobrazit plný text záznamu