Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

Autor: Lucila Traverso, Ivana Sierra, Sheila Ons, Hector Mario Masuh, Paula Valeria Gonzalez, Ariel Aptekmann, Alejandro D. Nadra, Jose Manuel Latorre-Estivalis
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Models
Molecular
0301 basic medicine
Life Cycles
Insecticides
Protein Conformation
Biología
RC955-962
Gene Expression
Disease Vectors
Toxicology
Pathology and Laboratory Medicine
Mosquitoes
Heat Shock Response
law.invention
Larvae
Medical Conditions
0302 clinical medicine
Aedes aegypti
Cytochrome P-450 Enzyme System
Aedes
law
Arctic medicine. Tropical medicine
Invertebrate Genomics
Medicine and Health Sciences
Heat-Shock Proteins
Cellular Stress Responses
Genetics
Eucalyptus
biology
Eukaryota
Agriculture
Genomics
Insects
Molecular Docking Simulation
Mosquito control
Infectious Diseases
Cell Processes
Essential oils
Larva
Insect Proteins
Public aspects of medicine
RA1-1270
Detoxification
Agrochemicals
Research Article
Arthropoda
030231 tropical medicine
Context (language use)
Arbovirus
03 medical and health sciences
Larvicidal activity
Oils
Volatile
medicine
Animals
Plant Oils
Gene family
Larvicide
Essential oil
Base Sequence
fungi
Organisms
Public Health
Environmental and Occupational Health
Biology and Life Sciences
Computational Biology
Cell Biology
biology.organism_classification
medicine.disease
Invertebrates
Insect Vectors
Species Interactions
030104 developmental biology
Eucalyptus camaldulensis
Gene Expression Regulation
Animal Genomics
RNA
Transcriptome
Zoology
Entomology
Developmental Biology
Zdroj: PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009587 (2021)
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
PLoS Neglected Tropical Diseases
Popis: Background: Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings: Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance: Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.
Los datos de la investigación están disponibles en la base de datos NCBI SRA (número de acceso Bioproject PRJNA671513) (link en "Documentos relacionados")
Centro Regional de Estudios Genómicos
Databáze: OpenAIRE
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