Optimisation of bovine β-lactoglobulin hydrolysis using cardosins from dried flowers of Cynara cardunculus
| Autor: | Pablo Fuciños, Clara Fuciños, Natalia Estévez, María L. Rúa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Whey protein
animal structures Proteolysis Ultrafiltration Cynara Flowers Lactoglobulins 01 natural sciences Antioxidants Substrate Specificity Analytical Chemistry Hydrolysis 0404 agricultural biotechnology medicine Animals Aspartic Acid Endopeptidases Chromatography High Pressure Liquid Plant Proteins chemistry.chemical_classification Chromatography Reverse-Phase Chromatography biology medicine.diagnostic_test 010401 analytical chemistry food and beverages Substrate (chemistry) 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences Diafiltration Enzyme chemistry Lactalbumin Cattle Food Science |
| Zdroj: | Food Chemistry. 345:128741 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2020.128741 |
| Popis: | Bovine whey protein was hydrolysed using cardosins A and B purified from dried flowers of Cynara cardunculus by combining diafiltration, anion-exchange chromatography and ultrafiltration. The proteolysis experiments were performed using different whey protein concentrations and enzyme/substrate (E/S) ratios. Complete hydrolysis of the main whey proteins, β-Lactoglobulin (β-Lg) and α-lactalbumin (α-La), was achieved after 4 h, at E/S ratios of 1/150 U/mg, regardless the initial protein concentration. In previous reports, the authors suggested that cardosins could not hydrolyse β-lactoblogulin. However, our promising results open up new possibilities to further explore the action of cardosins on whey proteins for the production of bioactive peptides. |
| Databáze: | OpenAIRE |
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