Herpes Simplex Virus Type 2 (HSV-2) Western Blot Confirmatory Testing Among Men Testing Positive for HSV-2 Using the Focus Enzyme-Linked Immunosorbent Assay in a Sexually Transmitted Disease Clinic
| Autor: | Rhoda Ashley-Morrow, Wayne R Hogrefe, Anna Wald, Paul D. Swenson, Matthew R. Golden, H. Hunter Handsfield |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Microbiology (medical) Sexually transmitted disease Adolescent genetic structures Herpesvirus 2 Human viruses Blotting Western Population Sexually Transmitted Diseases Enzyme-Linked Immunosorbent Assay Dermatology Antibodies Viral medicine.disease_cause Ambulatory Care Facilities Sensitivity and Specificity Herpesviridae Virus Predictive Value of Tests Alphaherpesvirinae Prevalence medicine Humans education Aged education.field_of_study Herpes Genitalis biology business.industry Public Health Environmental and Occupational Health Middle Aged biology.organism_classification Virology Infectious Diseases Herpes simplex virus Immunology biology.protein Viral disease Antibody business Algorithms |
| Zdroj: | Sexually Transmitted Diseases. 32:771-777 |
| ISSN: | 0148-5717 |
| Popis: | The objective of this study was to define the positive predictive value (PPV) of the Focus herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assay (ELISA) in a low HSV-2 prevalence population and to develop a new test interpretation algorithm.HSV-2 Western blots were performed on sera from male sexually transmitted disease clinic patients testing HSV-2 ELISA-positive and used to define a new class of indeterminate HSV-2 ELISA result. HSV-2 Western blots were then prospectively performed on sequential sera with indeterminate HSV-2 ELISAs.Ninety-one (84%) of 108 HSV-2 ELISA-positive sera tested HSV-2 Western blot-positive. Western blot positivity was more common in men without herpes simplex virus type 1 (HSV-1) antibody than in those with HSV-1 antibody (93% vs 76%, P = 0.02) and in men with a history or clinical evidence of genital lesions (88% vs 80%, P = 0.30). Selectively raising the ELISA index value defining HSV-2 positivity from1.1 toor=3.0 either among HSV-1-positive men or among those without a history or clinical evidence of genital lesions increased the PPV toor=93%. Prospective evaluation of an algorithm incorporating HSV-1 serostatus found that 11 of 70 persons with indeterminate HSV-2 ELISAs were Western blot-positive.Clinicians should consider selectively using a higher index value to define Focus ELISA HSV-2 positivity based on either HSV-1 serostatus or clinical circumstances. |
| Databáze: | OpenAIRE |
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