Real-Time, In Vivo Determination of Dynamic Changes in Lung and Heart Tissue Oxygenation Using EPR Oximetry
| Autor: | Matthew E. Joseph, Huagang Hou, Brian K. Rivera, Shan Naidu, Periannan Kuppusamy, Kamal Subramanian, Harold M. Swartz, Nadeem Khan |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Analytical chemistry chemistry.chemical_element Oxygen Article law.invention Rats Sprague-Dawley law medicine Animals Dimethylpolysiloxanes Oximetry Respiratory system Penetration depth Electron paramagnetic resonance Lung Myocardium Electron Spin Resonance Spectroscopy Partial pressure Rats Coupling (electronics) medicine.anatomical_structure chemistry Oxygen sensor Biomedical engineering |
| Zdroj: | Advances in Experimental Medicine and Biology ISBN: 9781493905836 |
| DOI: | 10.1007/978-1-4939-0620-8_11 |
| Popis: | The use of electron paramagnetic resonance (EPR) oximetry for oxygen measurements in deep tissues (>1 cm) is challenging due to the limited penetration depth of the microwave energy. To overcome this limitation, implantable resonators, having a small (0 5 mm diameter) sensory loop containing the oxygen-sensing paramagnetic material connected by a pair of twisted copper wire to a coupling loop (8–10 mm diameter), have been developed, which enable repeated measurements of deep-tissue oxygen levels (pO2, partial pressure of oxygen) in the brain and tumors of rodents. In this study, we have demonstrated the feasibility of measuring dynamic changes in pO2 in the heart and lung of rats using deep-tissue implantable oxygen sensors. The sensory loop of the resonator contained lithium octa-n-butoxynaphthalocyanine (LiNc-BuO) crystals embedded in polydimethylsiloxane (PDMS) polymer and was implanted in the myocardial tissue or lung pleura. The external coupling loop was secured subcutaneously above chest. The rats were exposed to different breathing gas mixtures while undergoing EPR measurements. The results demonstrated that implantable oxygen sensors provide reliable measurements of pO2 in deep tissues such as heart and lung under adverse conditions of cardiac and respiratory motions. |
| Databáze: | OpenAIRE |
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