Changes in radioimmunoassayable luteinizing hormone-releasing hormone in discrete brain areas of the rat at various times on proestrus, diestrous day 1, and after phenobarbital administration
| Autor: | Michael Selmanoff, Naomi Rance, Charles A. Barraclough, Phyllis M. Wise |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Anterior hypothalamic nucleus Radioimmunoassay Biology Gonadotropin-Releasing Hormone Endocrinology Estrus Arcuate nucleus Pregnancy Internal medicine medicine Animals Brain Chemistry Estradiol Suprachiasmatic nucleus Brain Diestrus Luteinizing Hormone Circadian Rhythm Rats Median eminence Phenobarbital Female Proestrus Gonadotropin Follicle Stimulating Hormone Luteinizing hormone hormones hormone substitutes and hormone antagonists Hormone medicine.drug |
| Zdroj: | Endocrinology. 108(6) |
| ISSN: | 0013-7227 |
| Popis: | Specific areas of the preoptico-suprachiasmatictuberoinfundibular system (PSTS) were microdissected at various hours on proestrus and diestrous day 1 in rats. LHRH concentrations were measured by RIA to determine if the patterns of change observed in the median eminence (ME) were paralleled by similar LHRH patterns in the remainder of the PSTS. Another group of proestrous rats received phenobarbital to block preovulatory gonadotropin surges, and LHRH concentrations in the PSTS were measured throughout the afternoon hours. Plasma concentrations of LH, FSH, estradiol, and progesterone were determined in all animals. Rats were decapitated on proestrus and diestrous day 1 at 0900, 1200,1500, and 1800 h. Proestrous animals that received phenobarbital at noon were sacrificed at 1500 and 1800 h. The following brain areas were microdissected: the suprachiasmatic preoptic nucleus, medial preoptic nucleus, suprachiasmatic nucleus, anterior hypothalamic nucleus retrochiasmatic area, arcuate nucleus, and ME. On the d... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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